このアイテムのアクセス数: 22

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
S2052252517008557.pdf2.02 MBAdobe PDF見る/開く
タイトル: Experimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography
著者: Yamashita, Keitaro
Kuwabara, Naoyuki
Nakane, Takanori
Murai, Tomohiro
Mizohata, Eiichi
Sugahara, Michihiro
Pan, Dongqing
Masuda, Tetsuya
Suzuki, Mamoru
Sato, Tomomi
Kodan, Atsushi
Yamaguchi, Tomohiro
Nango, Eriko
Tanaka, Tomoyuki
Tono, Kensuke
Joti, Yasumasa
Kameshima, Takashi
Hatsui, Takaki
Yabashi, Makina
Manya, Hiroshi
Endo, Tamao
Kato, Ryuichi
Senda, Toshiya
Kato, Hiroaki
Iwata, So
Ago, Hideo
Yamamoto, Masaki
Yumoto, Fumiaki
Nakatsu, Toru
著者名の別形: 山下, 恵太郎
桑原, 直之
中根, 崇智
村井, 智洋
溝端, 栄一
潘, 東青
鈴木, 守
登野, 健介
加藤, 博章
岩田, 想
湯本, 史明
中津, 亨
キーワード: serial femtosecond crystallography
SAD phasing
XFELs
selenomethionine derivatization
mercury soaking
発行日: Sep-2017
出版者: International Union of Crystallography (IUCr)
誌名: IUCrJ
巻: 4
号: 5
抄録: Serial femtosecond crystallography (SFX) using X-ray free-electron lasers (XFELs) holds enormous potential for the structure determination of proteins for which it is difficult to produce large and high-quality crystals. SFX has been applied to various systems, but rarely to proteins that have previously unknown structures. Consequently, the majority of previously obtained SFX structures have been solved by the molecular replacement method. To facilitate protein structure determination by SFX, it is essential to establish phasing methods that work efficiently for SFX. Here, selenomethionine derivatization and mercury soaking have been investigated for SFX experiments using the high-energy XFEL at the SPring-8 Angstrom Compact Free-Electron Laser (SACLA), Hyogo, Japan. Three successful cases are reported of single-wavelength anomalous diffraction (SAD) phasing using X-rays of less than 1 Å wavelength with reasonable numbers of diffraction patterns (13 000, 60 000 and 11 000). It is demonstrated that the combination of high-energy X-rays from an XFEL and commonly used heavy-atom incorporation techniques will enable routine de novo structural determination of biomacromolecules.
記述: SACLAの得意とするX線波長でタンパク質微結晶の新規構造解析に成功. 京都大学プレスリリース. 2017-08-23.
著作権等: This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
URI: http://hdl.handle.net/2433/226830
DOI(出版社版): 10.1107/S2052252517008557
関連リンク: http://www.kyoto-u.ac.jp/ja/research/research_results/2017/170810_2.html
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。