ダウンロード数: 183
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
s12967-016-1034-2.pdf | 4.19 MB | Adobe PDF | 見る/開く |
タイトル: | Immunogenic FEAT protein circulates in the bloodstream of cancer patients |
著者: | Li, Yan Kobayashi, Kyosuke Mona, Marwa M. Satomi, Chikako Okano, Shinji Inoue, Hiroyuki Tani, Kenzaburo Takahashi, Atsushi |
キーワード: | FEAT METTL13 Tumor marker ELISA Cancer screening Cancer prevention Immunotherapy |
発行日: | 22-Sep-2016 |
出版者: | Springer Nature |
誌名: | Journal of Translational Medicine |
巻: | 14 |
論文番号: | 275 |
抄録: | [Background]FEAT is an intracellular protein that potently drives tumorigenesis in vivo. It is only weakly expressed in normal human tissues, including the testis. In contrast, FEAT is aberrantly upregulated in most human cancers. The present study was designed to investigate whether FEAT is applicable to tumor immunotherapy and whether FEAT is discernible in the bloodstream as a molecular biomarker of human cancers. [Methods]Two mouse FEAT peptides with predicted affinities for major histocompatibility complex H-2Kb and H-2Db were injected subcutaneously into C57BL/6 mice before subcutaneous transplantation of isogenic B16-F10 melanoma cells. Intracellular localization of FEAT was determined by immunogold electron microscopy. Immunoprecipitation was performed to determine whether FEAT was present in blood from cancer patients. A sandwich enzyme-linked immunosorbent assay was used to measure FEAT concentrations in plasma from 30 cancer patients and eight healthy volunteers. [Results]The vaccination experiments demonstrated that FEAT was immunogenic, and that immune responses against FEAT were induced without deleterious side effects in mice. Electron microscopy revealed localization of FEAT in the cytoplasm, mitochondria, and nucleus. Immunoprecipitation identified FEAT in the blood plasma from cancer patients, while FEAT was not detected in plasma exosomes. Plasma FEAT levels were significantly higher in the presence of cancers. [Conclusions]These findings suggest that FEAT is a candidate for applications in early diagnosis and prevention of some cancers. |
著作権等: | © The Author(s) 2016 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
URI: | http://hdl.handle.net/2433/227066 |
DOI(出版社版): | 10.1186/s12967-016-1034-2 |
PubMed ID: | 27659353 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。