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Title: Association of lymph-node antigens with lower Gag-specific central-memory and higher Env-specific effector-memory CD8+ T-cell frequencies in a macaque AIDS model
Authors: Ishii, Hiroshi
Matsuoka, Saori
Nomura, Takushi
Nakamura, Midori
Shiino, Teiichiro
Sato, Yuko
Iwata-Yoshikawa, Naoko
Hasegawa, Hideki
Mizuta, Kazuta
Sakawaki, Hiromi
Miura, Tomoyuki  kyouindb  KAKEN_id
Koyanagi, Yoshio  kyouindb  KAKEN_id
Naruse, Taeko K.
Kimura, Akinori
Matano, Tetsuro
Author's alias: 阪脇, 廣美
三浦, 智行
小柳, 義夫
Keywords: HIV infections
Viral host response
Viral immune evasion
Issue Date: 25-Jul-2016
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 6
Thesis number: 30153
Abstract: Virus-specific CD8+ T cells exert strong suppressive pressure on human/simian immunodeficiency virus (HIV/SIV) replication. These responses have been intensively examined in peripheral blood mononuclear cells (PBMCs) but not fully analyzed in lymph nodes (LNs), where interaction between CD8[+] T cells and HIV/SIV-infected cells occurs. Here, we investigated target antigen specificity of CD8[+] T cells in LNs in a macaque AIDS model. Analysis of virus antigen-specific CD8[+] T-cell responses in the inguinal LNs obtained from twenty rhesus macaques in the chronic phase of SIV infection showed an inverse correlation between viral loads and frequencies of CD8[+] T cells with CD28[+]CD95[+] central memory phenotype targeting the N-terminal half of SIV core antigen (Gag-N). In contrast, analysis of LNs but not PBMCs revealed a positive correlation between viral loads and frequencies of CD8[+] T cells with CD28[−]CD95[+] effector memory phenotype targeting the N-terminal half of SIV envelope (Env-N), soluble antigen. Indeed, LNs with detectable SIV capsid p27 antigen in the germinal center exhibited significantly lower Gag-N-specific CD28[+] CD95[+] CD8[+] T-cell and higher Env-N-specific CD28[−]CD95[+] CD8[+] T-cell responses than those without detectable p27. These results imply that core and envelope antigen-specific CD8[+] T cells show different patterns of interactions with HIV/SIV-infected cells.
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
URI: http://hdl.handle.net/2433/227143
DOI(Published Version): 10.1038/srep30153
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