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Title: Human pluripotent stem cell–derived erythropoietin-producing cells ameliorate renal anemia in mice
Other Titles: Human pluripotent stem cell-derived erythropoietin-producing cells improve renal anemia in mice
Authors: Hitomi, Hirofumi
Kasahara, Tomoko
Katagiri, Naoko
Hoshina, Azusa
Mae, Shin-Ichi
Kotaka, Maki
Toyohara, Takafumi
Rahman, Asadur
Nakano, Daisuke
Niwa, Akira  kyouindb  KAKEN_id
Saito, Megumu K.
Nakahata, Tatsutoshi
Nishiyama, Akira
Osafune, Kenji  kyouindb  KAKEN_id
Author's alias: 人見, 浩史
西山, 成
長船, 健二
Issue Date: 27-Sep-2017
Publisher: American Association for the Advancement of Science (AAAS)
Journal title: Science Translational Medicine
Volume: 9
Issue: 409
Thesis number: eaaj2300
Abstract: The production of erythropoietin (EPO) by the kidneys, a principal hormone for the hematopoietic system, is reduced in patients with chronic kidney disease (CKD), eventually resulting in severe anemia. Although recombinant human EPO treatment improves anemia in patients with CKD, returning to full red blood cell production without fluctuations does not always occur. We established a method to generate EPO-producing cells from human induced pluripotent stem cells (hiPSCs) by modifying previously reported hepatic differentiation protocols. These cells showed increased EPO expression and secretion in response to low oxygen conditions, prolyl hydroxylase domain–containing enzyme inhibitors, and insulin-like growth factor 1. The EPO protein secreted from hiPSC-derived EPO-producing (hiPSC-EPO) cells induced the erythropoietic differentiation of human umbilical cord blood progenitor cells in vitro. Furthermore, transplantation of hiPSC-EPO cells into mice with CKD induced by adenine treatment improved renal anemia. Thus, hiPSC-EPO cells may be a useful tool for clarifying the mechanisms of EPO production and may be useful as a therapeutic strategy for treating renal anemia.
Description: ヒトiPS/ES細胞からエリスロポエチン産生細胞の作製に成功 --腎性貧血に対する細胞療法の可能性--. 京都大学プレスリリース. 2017-09-28.
Title of the accepted manuscript is 'Human pluripotent stem cell-derived erythropoietin-producing cells improve renal anemia in mice'.
Rights: This is the author's version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in 'Science Translational Medicine' on Vol. 9, Issue 409, eaaj2300, DOI: 10.1126/scitranslmed.aaj2300
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1126/scitranslmed.aaj2300
PubMed ID: 28954928
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