ダウンロード数: 260
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.stemcr.2017.09.007.pdf | 6.63 MB | Adobe PDF | 見る/開く |
タイトル: | Human Pluripotent Stem Cell-Derived Cardiac Tissue-like Constructs for Repairing the Infarcted Myocardium |
著者: | Li, Junjun Minami, Itsunari Shiozaki, Motoko Yu, Leqian Yajima, Shin Miyagawa, Shigeru Shiba, Yuji Morone, Nobuhiro Fukushima, Satsuki Yoshioka, Momoko Li, Sisi Qiao, Jing Li, Xin Wang, Lin Kotera, Hidetoshi Nakatsuji, Norio Sawa, Yoshiki Chen, Yong Liu, Li |
著者名の別形: | 李, 俊君 南, 一成 澤, 芳樹 陳, 勇 劉, 莉 |
キーワード: | cardiomyocytes human induced pluripotent stem cells cardiac tissue-like constructs myocardial infarction nanofiber extracellular recording drug assessment coupling engraftment arrhythmia |
発行日: | 14-Nov-2017 |
出版者: | Elsevier BV |
誌名: | Stem Cell Reports |
巻: | 9 |
号: | 5 |
開始ページ: | 1546 |
終了ページ: | 1559 |
抄録: | High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically and functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained high-quality cardiac tissue-like constructs (CTLCs) by cultivating hiPSC-CMs on low-thickness aligned nanofibers made of biodegradable poly(D, L-lactic-co-glycolic acid) polymer. We show that multilayered and elongated CMs could be organized at high density along aligned nanofibers in a simple one-step seeding process, resulting in upregulated cardiac biomarkers and enhanced cardiac functions. When used for drug assessment, CTLCs were much more robust than the 2D conventional control. We also demonstrated the potential of CTLCs for modeling engraftments in vitro and treating myocardial infarction in vivo. Thus, we established a handy framework for cardiac tissue engineering, which holds high potential for pharmaceutical and clinical applications. |
記述: | 新規ナノファイバー・iPS細胞由来心筋組織片を用いた再生医療技術の開発 --安全・配向・3次元心筋組織の構築に成功--. 京都大学プレスリリース. 2017-10-30. |
著作権等: | © 2017 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/227734 |
DOI(出版社版): | 10.1016/j.stemcr.2017.09.007 |
PubMed ID: | 29107590 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-10-30 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。