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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41598-017-08869-z.pdf | 2.98 MB | Adobe PDF | 見る/開く |
| タイトル: | Enhanced Therapeutic Effects of Human iPS Cell Derived-Cardiomyocyte by Combined Cell-Sheets with Omental Flap Technique in Porcine Ischemic Cardiomyopathy Model |
| 著者: | Kawamura, Masashi Miyagawa, Shigeru Fukushima, Satsuki Saito, Atsuhiro Miki, Kenji  Funakoshi, Shunsuke Yoshida, Yoshinori   https://orcid.org/0000-0001-5511-9090 (unconfirmed)Yamanaka, Shinya Shimizu, Tatsuya Okano, Teruo Daimon, Takashi Toda, Koichi Sawa, Yoshiki |
| 著者名の別形: | 舟越, 俊介 吉田, 善紀 山中, 伸弥 |
| キーワード: | Cardiomyopathies Heart failure |
| 発行日: | 18-Aug-2017 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 7 |
| 論文番号: | 8824 |
| 抄録: | Transplant of human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) cell-sheet is a promising approach for treating ischemic cardiomyopathy (ICM). However, poor blood supply to the transplanted cell-sheet is a concern related to the effectiveness and durability of the treatment. Herein, we hypothesized that the combined the omentum flap might enhance survival and the therapeutic effects of hiPS-CM cell-sheet transplant for ICM treatment. Treatment by Wnt signaling molecules in hiPS cells produced hiPS-CMs, which were magnetically labeled by superparamagnetic iron oxide (SPIO), followed by culture in the thermoresponsive dishes to generate hiPS-CMs cell-sheets. A porcine ICM model included 4 groups; sham operation, omentum flap only, cell-sheet only, or combination therapy. Ejection fraction (EF) was significantly greater in the cell-sheet only and combination group compared to the other groups during the follow-up period. At 3 months, the EF of the combination group was significantly greater than that of the cell-sheet only group. Consistently, the survival rate of the SPIO-labeled hiPS-CMs, as assessed by MRI, was significantly greater in the combination group than in the cell-sheet only group. This cell delivery system would be useful in optimizing the hiPS-CM cell-sheet transplant for treating severe heart failure. |
| 著作権等: | © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
| URI: | http://hdl.handle.net/2433/228151 |
| DOI(出版社版): | 10.1038/s41598-017-08869-z |
| PubMed ID: | 28821761 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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