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Title: Enhanced Therapeutic Effects of Human iPS Cell Derived-Cardiomyocyte by Combined Cell-Sheets with Omental Flap Technique in Porcine Ischemic Cardiomyopathy Model
Authors: Kawamura, Masashi
Miyagawa, Shigeru
Fukushima, Satsuki
Saito, Atsuhiro
Miki, Kenji  kyouindb  KAKEN_id
Funakoshi, Shunsuke
Yoshida, Yoshinori  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5511-9090 (unconfirmed)
Yamanaka, Shinya  kyouindb  KAKEN_id
Shimizu, Tatsuya
Okano, Teruo
Daimon, Takashi
Toda, Koichi
Sawa, Yoshiki
Author's alias: 舟越, 俊介
吉田, 善紀
山中, 伸弥
Keywords: Cardiomyopathies
Heart failure
Issue Date: 18-Aug-2017
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 7
Thesis number: 8824
Abstract: Transplant of human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) cell-sheet is a promising approach for treating ischemic cardiomyopathy (ICM). However, poor blood supply to the transplanted cell-sheet is a concern related to the effectiveness and durability of the treatment. Herein, we hypothesized that the combined the omentum flap might enhance survival and the therapeutic effects of hiPS-CM cell-sheet transplant for ICM treatment. Treatment by Wnt signaling molecules in hiPS cells produced hiPS-CMs, which were magnetically labeled by superparamagnetic iron oxide (SPIO), followed by culture in the thermoresponsive dishes to generate hiPS-CMs cell-sheets. A porcine ICM model included 4 groups; sham operation, omentum flap only, cell-sheet only, or combination therapy. Ejection fraction (EF) was significantly greater in the cell-sheet only and combination group compared to the other groups during the follow-up period. At 3 months, the EF of the combination group was significantly greater than that of the cell-sheet only group. Consistently, the survival rate of the SPIO-labeled hiPS-CMs, as assessed by MRI, was significantly greater in the combination group than in the cell-sheet only group. This cell delivery system would be useful in optimizing the hiPS-CM cell-sheet transplant for treating severe heart failure.
Rights: © The Author(s) 2017.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
URI: http://hdl.handle.net/2433/228151
DOI(Published Version): 10.1038/s41598-017-08869-z
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