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Title: The CDK-PLK1 axis targets the DNA damage checkpoint sensor protein RAD9 to promote cell proliferation and tolerance to genotoxic stress
Authors: Wakida, Takeshi
Ikura, Masae
Kuriya, Kenji
Ito, Shinji
Shiroiwa, Yoshiharu
Habu, Toshiyuki
Kawamoto, Takuo  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-0775-0491 (unconfirmed)
Okumura, Katsuzumi
Ikura, Tsuyoshi  kyouindb  KAKEN_id
Furuya, Kanji  kyouindb  KAKEN_id
Author's alias: 井倉, 正枝
井倉, 毅
古谷, 寛治
Issue Date: 19-Dec-2017
Publisher: eLife Sciences Organisation, Ltd.
Journal title: eLife
Volume: 6
Thesis number: e29953
Abstract: Genotoxic stress causes proliferating cells to activate the DNA damage checkpoint, to assist DNA damage recovery by slowing cell cycle progression. Thus, to drive proliferation, cells must tolerate DNA damage and suppress the checkpoint response. However, the mechanism underlying this negative regulation of checkpoint activation is still elusive. We show that human Cyclin-Dependent-Kinases (CDKs) target the RAD9 subunit of the 9-1-1 checkpoint clamp on Thr292, to modulate DNA damage checkpoint activation. Thr292 phosphorylation on RAD9 creates a binding site for Polo-Like-Kinase1 (PLK1), which phosphorylates RAD9 on Thr313. These CDK-PLK1-dependent phosphorylations of RAD9 suppress checkpoint activation, therefore maintaining high DNA synthesis rates during DNA replication stress. Our results suggest that CDK locally initiates a PLK1-dependent signaling response that antagonizes the ability of the DNA damage checkpoint to detect DNA damage. These findings provide a mechanism for the suppression of DNA damage checkpoint signaling, to promote cell proliferation under genotoxic stress conditions.
Description: 二刀流のがん増殖戦略. 京都大学プレスリリース. 2017-12-20.
Rights: © 2017, Wakida et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
URI: http://hdl.handle.net/2433/228267
DOI(Published Version): 10.7554/eLife.29953
PubMed ID: 29254517
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2017/171219_1.html
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