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Title: Age-dependent deterioration of locomotion inDrosophila melanogasterdeficient in the homologue ofamyotrophic lateral sclerosis 2
Authors: Takayama, Yuta
Itoh, Reina E.
Tsuyama, Taiichi
Uemura, Tadashi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7204-3606 (unconfirmed)
Author's alias: 高山, 雄太
伊藤, 玲奈
津山, 泰一
上村, 匡
Issue Date: Jun-2014
Publisher: Wiley-Blackwell
Journal title: Genes to Cells
Volume: 19
Issue: 6
Start page: 464
End page: 477
Abstract: Recessive mutations in the amyotrophic lateral sclerosis 2 (ALS2) gene have been linked to juvenile-onset ALS2. Although one of the molecular functions of the ALS2 protein is clearly the activation of Rab5, the mechanisms underlying the selective dysfunction and degeneration of motor neurons in vivo remain to be fully understood. Here, we focused on the ALS2 homologue of Drosophila melanogaster, isolated two independent deletions, and systematically compared phenotypes of the mutants with those of animals in which Rab5 function in identified neurons was abrogated. In the dALS2 mutant flies, we found that the stereotypic axonal and dendritic morphologies of neurons shared some features with those in Rab5-deficient flies, but the dALS2 mutant phenotypes were much milder. We also found that the abrogation of Rab5 function in motor neurons strongly depressed the locomotion activity of adults, resembling the behavior of aged dALS2 mutants. Importantly, this age-dependent locomotion deficit of dALS2 mutants was restored to normal by expressing the dALS2 transgene in a wide range of tissues. This finding provided a platform where we could potentially identify particular cell types responsible for the phenotype by tissue-specific rescue experiments. We discuss our results and the future usage of the dALS2 mutant as a new ALS model.
Rights: This is the accepted version of the following article: [Takayama, Y., Itoh, R. E., Tsuyama, T., Uemura, T. (2014), Age-dependent deterioration of locomotion in Drosophila melanogaster deficient in the homologue of amyotrophic lateral sclerosis 2. Genes to Cells, 19: 464–477], which has been published in final form at https://doi.org/10.1111/gtc.12146. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
The full-text file will be made open to the public on 23 MAY 2015 in accordance with publisher's 'Terms and Conditions for Self-Archiving'
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/230355
DOI(Published Version): 10.1111/gtc.12146
PubMed ID: 24702731
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