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タイトル: | Insulin secretion of mixed insulinoma aggregates-gelatin hydrogel microspheres after subcutaneous transplantation |
著者: | Inoo, Kanako Bando, Hiroto Tabata, Yasuhiko |
著者名の別形: | 田畑, 泰彦 |
キーワード: | Insulinoma cel Aggregate Gelatin microsphere Vascularization Glucose-induced insulin secretion |
発行日: | 1-Jun-2018 |
出版者: | Elsevier BV |
誌名: | Regenerative Therapy |
巻: | 8 |
開始ページ: | 38 |
終了ページ: | 45 |
抄録: | Introduction: The objective of this study is to evaluate the insulin secretion of mixed aggregates of insulinoma cells (INS-1) and gelatin hydrogel microspheres after their subcutaneous transplantation. Methods: Gelatin hydrogel microspheres were prepared by the conventional w/o emulsion method. Cell aggregates mixed with or without the hydrogel microspheres were encapsulated into a pouched-device of polytetrafluoroethylene membrane. An agarose hydrogel or MedGel™ incorporating basic fibroblast growth factor (bFGF) was subcutaneously implanted to induce vascularization. After the vascularization induction, cell aggregates encapsulated in the pouched-device was transplanted. Results: The vascularization had the potential to enable transplanted cell aggregates to enhance the level of insulin secretion compared with those of no vascularization induction. In addition, the insulin secretion of cell aggregates was significantly promoted by the mixing of gelatin hydrogel microspheres even in the pouched-device encapsulated state. Conclusion: It is possible that the microspheres mixing gives cells in aggregates better survival condition, resulting in promoted insulin secretion. |
著作権等: | © 2018, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0). |
URI: | http://hdl.handle.net/2433/230357 |
DOI(出版社版): | 10.1016/j.reth.2018.01.003 |
PubMed ID: | 30271864 |
出現コレクション: | 学術雑誌掲載論文等 |
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