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タイトル: | Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor |
著者: | Sato, Keita Yamashita, Takahiro Ohuchi, Hideyo Takeuchi, Atsuko Gotoh, Hitoshi Ono, Katsuhiko Mizuno, Misao https://orcid.org/0000-0003-2206-1763 (unconfirmed) Mizutani, Yasuhisa Tomonari, Sayuri Sakai, Kazumi Imamoto, Yasushi https://orcid.org/0000-0002-0803-4163 (unconfirmed) Wada, Akimori Shichida, Yoshinori |
著者名の別形: | 佐藤, 恵太 山下, 高廣 大内, 淑代 七田, 芳則 |
キーワード: | Biochemistry G protein-coupled receptors |
発行日: | 28-Mar-2018 |
出版者: | Springer Nature |
誌名: | Nature Communications |
巻: | 9 |
論文番号: | 1255 |
抄録: | Most opsins are G protein-coupled receptors that utilize retinal both as a ligand and as a chromophore. Opsins’ main established mechanism is light-triggered activation through retinal 11-cis-to-all-trans photoisomerization. Here we report a vertebrate non-visual opsin that functions as a Gi-coupled retinal receptor that is deactivated by light and can thermally self-regenerate. This opsin, Opn5L1, binds exclusively to all-trans-retinal. More interestingly, the light-induced deactivation through retinal trans-to-cis isomerization is followed by formation of a covalent adduct between retinal and a nearby cysteine, which breaks the retinal-conjugated double bond system, probably at the C11 position, resulting in thermal re-isomerization to all-trans-retinal. Thus, Opn5L1 acts as a reverse photoreceptor. We conclude that, like vertebrate rhodopsin, Opn5L1 is a unidirectional optical switch optimized from an ancestral bidirectional optical switch, such as invertebrate rhodopsin, to increase the S/N ratio of the signal transduction, although the direction of optimization is opposite to that of vertebrate rhodopsin. |
記述: | 光を受けるとオフになる動物のユニークな光センサーを発見 --ニワトリの脳内で機能するOpn5L1の性質を解明--. 京都大学プレスリリース. 2018-04-02. |
著作権等: | © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
URI: | http://hdl.handle.net/2433/230387 |
DOI(出版社版): | 10.1038/s41467-018-03603-3 |
PubMed ID: | 29593298 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2018-04-02-1 |
出現コレクション: | 学術雑誌掲載論文等 |
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