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j.stemcr.2018.03.005.pdf | 5.4 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Watanabe, Satoshi | en |
dc.contributor.author | Kanatsu-Shinohara, Mito | en |
dc.contributor.author | Ogonuki, Narumi | en |
dc.contributor.author | Matoba, Shogo | en |
dc.contributor.author | Ogura, Atsuo | en |
dc.contributor.author | Shinohara, Takashi | en |
dc.contributor.alternative | 渡邉, 哲史 | ja |
dc.contributor.alternative | 篠原, 美都 | ja |
dc.contributor.alternative | 越後貫, 成美 | ja |
dc.contributor.alternative | 的場, 章悟 | ja |
dc.contributor.alternative | 小倉, 淳郎 | ja |
dc.contributor.alternative | 篠原, 隆司 | ja |
dc.date.accessioned | 2018-04-10T07:39:28Z | - |
dc.date.available | 2018-04-10T07:39:28Z | - |
dc.date.issued | 2018-05-08 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/2433/230498 | - |
dc.description | セルトリ細胞への遺伝子導入による新規男性不妊治療法の開発 --ヒト不妊治療への応用に期待--. 京都大学プレスリリース. 2018-04-10. | ja |
dc.description.abstract | Adeno-associated virus (AAV) penetrates the blood-brain barrier, but it is unknown whether AAV penetrates other tight junctions. Genetic manipulation of testis has been hampered by the basement membrane of seminiferous tubules and the blood-testis barrier (BTB), which forms between Sertoli cells and divides the tubules into basal and adluminal compartments. Here, we demonstrate in vivo genetic manipulation of spermatogonial stem cells (SSCs) and their microenvironment via AAV1/9. AAV1/9 microinjected into the seminiferous tubules penetrated both the basement membrane and BTB, thereby transducing not only Sertoli cells and SSCs but also peritubular cells and Leydig cells. Moreover, when congenitally infertile KitlSl/KitlSl-d mouse testes with defective Sertoli cells received Kitl-expressing AAVs, spermatogenesis regenerated and offspring were produced. None of the offspring contained the AAV genome. Thus, AAV1/9 allows efficient germline and niche manipulation by penetrating the BTB and basement membrane, providing a promising strategy for the development of gene therapies for reproductive defects. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2018 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | en |
dc.subject | adeno-associated virus | en |
dc.subject | blood-testis barrier | en |
dc.subject | Sertoli cells | en |
dc.subject | spermatogonia | en |
dc.subject | spermatogenesis | en |
dc.title | In Vivo Genetic Manipulation of Spermatogonial Stem Cells and Their Microenvironment by Adeno-Associated Viruses | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Stem Cell Reports | en |
dc.identifier.volume | 10 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 1551 | - |
dc.identifier.epage | 1564 | - |
dc.relation.doi | 10.1016/j.stemcr.2018.03.005 | - |
dc.textversion | publisher | - |
dc.address | Department of Molecular Genetics, Graduate School of Medicine, Kyoto University | en |
dc.address | Department of Molecular Genetics, Graduate School of Medicine, Kyoto University | en |
dc.address | RIKEN, Bioresource Center | en |
dc.address | RIKEN, Bioresource Center | en |
dc.address | RIKEN, Bioresource Center | en |
dc.address | Department of Molecular Genetics, Graduate School of Medicine, Kyoto University | en |
dc.identifier.pmid | 29628393 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2018-04-10 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 16K18400 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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