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タイトル: | Evidence for ESCRT- and clathrin-dependent microautophagy |
著者: | Oku, Masahide Maeda, Yuichiro Kagohashi, Yoko Kondo, Takeshi Yamada, Mai Fujimoto, Toyoshi Sakai, Yasuyoshi |
著者名の別形: | 奥, 公秀 阪井, 康能 |
キーワード: | Metabolism Microbiology Organelles |
発行日: | Oct-2017 |
出版者: | Rockefeller University Press |
誌名: | Journal of Cell Biology |
巻: | 216 |
号: | 10 |
開始ページ: | 3263 |
終了ページ: | 3274 |
抄録: | Microautophagy refers to a mode of autophagy in which the lysosomal or vacuolar membrane invaginates and directly engulfs target components. The molecular machinery of membrane dynamics driving microautophagy is still elusive. Using immunochemical monitoring of yeast vacuolar transmembrane proteins, Vph1 and Pho8, fused to fluorescent proteins, we obtained evidence showing an induction of microautophagy after a diauxic shift in the yeast Saccharomyces cerevisiae. Components of the endosomal sorting complex required for transport machinery were found to be required for this process, and the gateway protein of the machinery, Vps27, was observed to change its localization onto the vacuolar membrane after a diauxic shift. We revealed the functional importance of Vps27’s interaction with clathrin in this microautophagy that also contributed to uptake of lipid droplets into the vacuole. This study sheds light on the molecular mechanism of microautophagy, which does not require the core Atg proteins. |
記述: | 大隅Atg分子を必要としないミクロオートファジーの新しいメカニズム. 京都大学プレスリリース. 2017-08-25. |
著作権等: | © 2017 Oku et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
URI: | http://hdl.handle.net/2433/230534 |
DOI(出版社版): | 10.1083/jcb.201611029 |
PubMed ID: | 28838958 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2017-08-25 |
出現コレクション: | 学術雑誌掲載論文等 |
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