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タイトル: | Reversible Morphological Control of Tubulin-Encapsulating Giant Liposomes by Hydrostatic Pressure |
著者: | Hayashi, Masahito Nishiyama, Masayoshi Kazayama, Yuki Toyota, Taro Harada, Yoshie Takiguchi, Kingo |
著者名の別形: | 林, 真人 西山, 雅祥 風山, 祐輝 豊田, 太郎 原田, 慶恵 滝口, 金吾 |
発行日: | 19-Apr-2016 |
出版者: | American Chemical Society (ACS) |
誌名: | Langmuir |
巻: | 32 |
号: | 15 |
開始ページ: | 3794 |
終了ページ: | 3802 |
抄録: | Liposomes encapsulating cytoskeletons have drawn much recent attention to develop an artificial cell-like chemical-machinery; however, as far as we know, there has been no report showing isothermally reversible morphological changes of liposomes containing cytoskeletons because the sets of various regulatory factors, that is, their interacting proteins, are required to control the state of every reaction system of cytoskeletons. Here we focused on hydrostatic pressure to control the polymerization state of microtubules (MTs) within cell-sized giant liposomes (diameters ∼10 μm). MT is the cytoskeleton formed by the polymerization of tubulin, and cytoskeletal systems consisting of MTs are very dynamic and play many important roles in living cells, such as the morphogenesis of nerve cells and formation of the spindle apparatus during mitosis. Using real-time imaging with a high-pressure microscope, we examined the effects of hydrostatic pressure on the morphology of tubulin-encapsulating giant liposomes. At ambient pressure (0.1 MPa), many liposomes formed protrusions due to tubulin polymerization within them. When high pressure (60 MPa) was applied, the protrusions shrank within several tens of seconds. This process was repeatedly inducible (around three times), and after the pressure was released, the protrusions regenerated within several minutes. These deformation rates of the liposomes are close to the velocities of migrating or shape-changing living cells rather than the shortening and elongation rates of the single MTs, which have been previously measured. These results demonstrate that the elongation and shortening of protrusions of giant liposomes is repeatedly controllable by regulating the polymerization state of MTs within them by applying and releasing hydrostatic pressure. |
記述: | 細胞サイズの人工膜小胞を可逆的に繰り返し変形させられることに成功 (生体運動マシナリーを使った分子ロボット構築の可能性を実証). 京都大学プレスリリース. 2016-04-19. |
著作権等: | © 2016 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
URI: | http://hdl.handle.net/2433/230561 |
DOI(出版社版): | 10.1021/acs.langmuir.6b00799 |
PubMed ID: | 27023063 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2016-04-19 |
出現コレクション: | 学術雑誌掲載論文等 |
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