ダウンロード数: 278
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
acs.biochem.7b01059.pdf | 3.19 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Obata, Shunsuke | en |
dc.contributor.author | Asamitsu, Sefan | en |
dc.contributor.author | Hashiya, Kaori | en |
dc.contributor.author | Bando, Toshikazu | en |
dc.contributor.author | Sugiyama, Hiroshi | en |
dc.contributor.alternative | 小幡, 俊介 | ja |
dc.contributor.alternative | 橋谷, かおり | ja |
dc.contributor.alternative | 板東, 俊和 | ja |
dc.contributor.alternative | 杉山, 弘 | ja |
dc.date.accessioned | 2018-04-25T06:47:16Z | - |
dc.date.available | 2018-04-25T06:47:16Z | - |
dc.date.issued | 2018-02-06 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.uri | http://hdl.handle.net/2433/230865 | - |
dc.description.abstract | The G-quadruplex (G4) is one type of higher-order structure of nucleic acids and is thought to play important roles in various biological events such as regulation of transcription and inhibition of DNA replication. Pyrrole–imidazole polyamides (PIPs) are programmable small molecules that can sequence-specifically bind with high affinity to the minor groove of double-stranded DNA (dsDNA). Herein, we designed head-to-head hairpin PIP dimers and their target dsDNA in a model G4-forming sequence. Using an electrophoresis mobility shift assay and transcription arrest assay, we found that PIP dimers could induce the structural change to G4 DNA from dsDNA through the recognition by one PIP dimer molecule of two duplex-binding sites flanking both ends of the G4-forming sequence. This induction ability was dependent on linker length. This is the first study to induce G4 formation using PIPs, which are known to be dsDNA binders. The results reported here suggest that selective G4 induction in native sequences may be achieved with PIP dimers by applying the same design strategy. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society | en |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in 'Biochemistry', copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.biochem. | en |
dc.rights | The full-text file will be made open to the public on 13 December 2018 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.title | G-Quadruplex Induction by the Hairpin Pyrrole-Imidazole Polyamide Dimer | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Biochemistry | en |
dc.identifier.volume | 57 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 498 | - |
dc.identifier.epage | 502 | - |
dc.relation.doi | 10.1021/acs.biochem.7b01059 | - |
dc.textversion | author | - |
dc.address | aDepartment of Chemistry, Graduate School of Science, Kyoto University | en |
dc.address | aDepartment of Chemistry, Graduate School of Science, Kyoto University | en |
dc.address | aDepartment of Chemistry, Graduate School of Science, Kyoto University | en |
dc.address | aDepartment of Chemistry, Graduate School of Science, Kyoto University | en |
dc.address | aDepartment of Chemistry, Graduate School of Science, Kyoto University・Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.identifier.pmid | 29236465 | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2018-12-13 | - |
出現コレクション: | 学術雑誌掲載論文等 |
![](/dspace/image/articlelinker.gif)
このリポジトリに保管されているアイテムはすべて著作権により保護されています。