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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| chem.201602435.pdf | 3.27 MB | Adobe PDF | 見る/開く |
| タイトル: | Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein |
| 著者: | Kizaki, Seiichiro Zou, Tingting Li, Yue Han, Yong Woon Suzuki, Yuki Harada, Yoshie Sugiyama, Hiroshi    https://orcid.org/0000-0001-8923-5946 (unconfirmed) |
| 著者名の別形: | 鈴木, 勇輝 原田, 慶恵 杉山, 弘 |
| キーワード: | AFM imaging cytosine DNA nucleosomes proteins |
| 発行日: | 7-Nov-2016 |
| 出版者: | Wiley-VCH Verlag |
| 誌名: | Chemistry - A European Journal |
| 巻: | 22 |
| 開始ページ: | 16598 |
| 終了ページ: | 16601 |
| 抄録: | Tet (ten-eleven translocation) family proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC), and are suggested to be involved in the active DNA demethylation pathway. In this study, we reconstituted positioned mononucleosomes using CpG-methylated 382bp DNA containing the Widom 601 sequence and recombinant histone octamer, and subjected the nucleosome to treatment with Tet1 protein. The sites of oxidized methylcytosine were identified by bisulfite sequencing. We found that, for the oxidation reaction, Tet1 protein prefers mCs located in the linker region of the nucleosome compared with those located in the core region. |
| 著作権等: | This is the accepted version of the following article: [Seiichiro Kizaki, Tingting Zou, Yue Li, Yong‐Woon Han, Yuki Suzuki, Yoshie Harada, Hiroshi Sugiyama. Preferential 5‐Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein. ChemBioChem (2016), 22, 46, 16598-16601], which has been published in final form at https://doi.org/10.1002/chem.201602435. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. The full-text file will be made open to the public on 02 November 2017 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
| URI: | http://hdl.handle.net/2433/230881 |
| DOI(出版社版): | 10.1002/chem.201602435 |
| PubMed ID: | 27689340 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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