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Title: Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein
Authors: Kizaki, Seiichiro
Zou, Tingting
Li, Yue
Han, Yong Woon
Suzuki, Yuki
Harada, Yoshie
Sugiyama, Hiroshi
Author's alias: 鈴木, 勇輝
原田, 慶恵
杉山, 弘
Keywords: AFM imaging
cytosine
DNA
nucleosomes
proteins
Issue Date: 7-Nov-2016
Publisher: Wiley-VCH Verlag
Journal title: Chemistry - A European Journal
Volume: 22
Start page: 16598
End page: 16601
Abstract: Tet (ten-eleven translocation) family proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC), and are suggested to be involved in the active DNA demethylation pathway. In this study, we reconstituted positioned mononucleosomes using CpG-methylated 382bp DNA containing the Widom 601 sequence and recombinant histone octamer, and subjected the nucleosome to treatment with Tet1 protein. The sites of oxidized methylcytosine were identified by bisulfite sequencing. We found that, for the oxidation reaction, Tet1 protein prefers mCs located in the linker region of the nucleosome compared with those located in the core region.
Rights: This is the accepted version of the following article: [Seiichiro Kizaki, Tingting Zou, Yue Li, Yong‐Woon Han, Yuki Suzuki, Yoshie Harada, Hiroshi Sugiyama. Preferential 5‐Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein. ChemBioChem (2016), 22, 46, 16598-16601], which has been published in final form at https://doi.org/10.1002/chem.201602435. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
The full-text file will be made open to the public on 02 November 2017 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/230881
DOI(Published Version): 10.1002/chem.201602435
PubMed ID: 27689340
Appears in Collections:Journal Articles

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