Access count of this item: 43
|Title:||Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein|
Han, Yong Woon
|Author's alias:||鈴木, 勇輝|
|Journal title:||Chemistry - A European Journal|
|Abstract:||Tet (ten-eleven translocation) family proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC), and are suggested to be involved in the active DNA demethylation pathway. In this study, we reconstituted positioned mononucleosomes using CpG-methylated 382bp DNA containing the Widom 601 sequence and recombinant histone octamer, and subjected the nucleosome to treatment with Tet1 protein. The sites of oxidized methylcytosine were identified by bisulfite sequencing. We found that, for the oxidation reaction, Tet1 protein prefers mCs located in the linker region of the nucleosome compared with those located in the core region.|
|Rights:||This is the accepted version of the following article: [Seiichiro Kizaki, Tingting Zou, Yue Li, Yong‐Woon Han, Yuki Suzuki, Yoshie Harada, Hiroshi Sugiyama. Preferential 5‐Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein. ChemBioChem (2016), 22, 46, 16598-16601], which has been published in final form at https://doi.org/10.1002/chem.201602435. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.|
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|Appears in Collections:||Journal Articles|
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