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タイトル: | CesA protein is included in the terminal complex of Acetobacter |
著者: | Sun, Shi-jing Imai, Tomoya https://orcid.org/0000-0001-7026-0287 (unconfirmed) Sugiyama, Junji https://orcid.org/0000-0002-5388-4925 (unconfirmed) Kimura, Satoshi |
著者名の別形: | 今井, 友也 杉山, 淳司 |
キーワード: | CesA Cellulose synthase complex Terminal complex Immunolabeling |
発行日: | May-2017 |
出版者: | Springer Nature |
誌名: | Cellulose |
巻: | 24 |
号: | 5 |
開始ページ: | 2017 |
終了ページ: | 2027 |
抄録: | Cellulose is a major biopolymer on the earth that is produced by cellulose synthase in the cell membrane of living organisms. Cellulose synthase is a hetero-subunit complex composed of several different protein subunits, and is visualized as a supermolecular complex called a “terminal complex” by electron microscopy. Such supermolecular organization of an enzyme complex is believed to be important for the fiber formation or crystallization of cellulose microfibrils in cellulose biosynthesis. In the case of the cellulose-producing bacterium Acetobacter, it is hypothesized that the enzyme complex includes at least six subunits given its genetic constitution. However, to date, only three of these molecules have been experimentally confirmed as the subunits included in the terminal complex: CesB, CesD, and ccp2. In this study, we used fluorescence immuno-microscopy to show that CesA protein, the catalytic subunit, is included in the terminal complex of Acetobacter. Furthermore we discuss the obtained microscopic data for improving our understanding of the molecular organization of the bacterial cellulose synthase complex. |
著作権等: | This is a post-peer-review, pre-copyedit version of an article published in 'Cellulose'. The final authenticated version is available online at: https://doi.org/10.1007/s10570-017-1237-6. The full-text file will be made open to the public on 1 May 2017 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/230960 |
DOI(出版社版): | 10.1007/s10570-017-1237-6 |
出現コレクション: | 学術雑誌掲載論文等 |
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