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Title: Adaptation of the Freshwater Bloom-Forming Cyanobacterium Microcystis aeruginosa to Brackish Water Is Driven by Recent Horizontal Transfer of Sucrose Genes
Authors: Tanabe, Yuuhiko
Hodoki, Yoshikuni
Sano, Tomoharu
Tada, Kiyoshi
Watanabe, Makoto M.
Author's alias: 田辺, 雄彦
程木, 義邦
佐野, 友春
多田, 清志
渡邉, 信
Keywords: Microcystis
bloom
salt tolerance
sucrose
genomics /brackish water
horizontal gene trasnfer
ecotype
Issue Date: 5-Jun-2018
Publisher: Frontiers Media SA
Journal title: Frontiers in Microbiology
Volume: 9
Thesis number: 1150
Abstract: Microcystis aeruginosa is a bloom-forming cyanobacterium found in eutrophic water bodies worldwide. M. aeruginosa blooms usually occur in freshwater; however, they have also been reported to occur in brackish water. Because M. aeruginosa often produces the cyanotoxin microcystin, they are a major concern to public health and environment. Despite this, the ecology, genomic basis, and evolutionary process underlying the M. aeruginosa bloom invasion from fresh to brackish water have been poorly investigated. Hence, in the present study, we have sequenced and characterized genomes of two newly discovered salt-tolerant M. aeruginosa strains obtained from Japanese brackish water lakes (Lakes Shinji and Tofutsu). Both genomes contain a set of genes for the synthesis of osmolyte sucrose (sppA, spsA, and susA), hitherto identified in only one strain (PCC 7806) of M. aeruginosa. Chemical and gene expression analyses confirmed sucrose accumulation induced by salt. A comprehensive genetic survey of >200 strains indicated that sucrose genes are extremely rare in M. aeruginosa. Most surprisingly, comparative genome analyses of the three strains indicated extremely low genetic diversity in the sucrose genes compared with other core genome genes, suggesting very recent acquisitions via horizontal transfer. Invasion of M. aeruginosa blooms into brackish water may be a recent event triggered by anthropogenic eutrophication of brackish water.
Description: 宍道湖、網走湖などの汽水湖でアオコが出現するメカニズムを解明しました. 京都大学プレスリリース. 2018-06-20.
Rights: © 2018 Tanabe, Hodoki, Sano, Tada and Watanabe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
URI: http://hdl.handle.net/2433/231981
DOI(Published Version): 10.3389/fmicb.2018.01150
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2018/180605_1.html
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