ダウンロード数: 152
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.stemcr.2018.05.020.pdf | 4.32 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Iezaki, Takashi | en |
dc.contributor.author | Horie, Tetsuhiro | en |
dc.contributor.author | Fukasawa, Kazuya | en |
dc.contributor.author | Kitabatake, Makoto | en |
dc.contributor.author | Nakamura, Yuka | en |
dc.contributor.author | Park, Gyujin | en |
dc.contributor.author | Onishi, Yuki | en |
dc.contributor.author | Ozaki, Kakeru | en |
dc.contributor.author | Kanayama, Takashi | en |
dc.contributor.author | Hiraiwa, Manami | en |
dc.contributor.author | Kitaguchi, Yuka | en |
dc.contributor.author | Kaneda, Katsuyuki | en |
dc.contributor.author | Manabe, Takayuki | en |
dc.contributor.author | Ishigaki, Yasuhito | en |
dc.contributor.author | Ohno, Mutsuhito | en |
dc.contributor.author | Hinoi, Eiichi | en |
dc.contributor.alternative | 北畠, 真 | ja |
dc.contributor.alternative | 大野, 睦人 | ja |
dc.date.accessioned | 2018-07-23T01:44:07Z | - |
dc.date.available | 2018-07-23T01:44:07Z | - |
dc.date.issued | 2018-07-10 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/2433/232693 | - |
dc.description.abstract | The mechanistic/mammalian target of rapamycin complex 1 (mTORC1) regulates cellular function in various cell types. Although the role of mTORC1 in skeletogenesis has been investigated previously, here we show a critical role of mTORC1/4E-BPs/SOX9 axis in regulating skeletogenesis through its expression in undifferentiated mesenchymal cells. Inactivation of Raptor, a component of mTORC1, in limb buds before mesenchymal condensations resulted in a marked loss of both cartilage and bone. Mechanistically, we demonstrated that mTORC1 selectively controls the RNA translation of Sox9, which harbors a 5′ terminal oligopyrimidine tract motif, via inhibition of the 4E-BPs. Indeed, introduction of Sox9 or a knockdown of 4E-BP1/2 in undifferentiated mesenchymal cells markedly rescued the deficiency of the condensation observed in Raptor-deficient mice. Furthermore, introduction of the Sox9 transgene rescued phenotypes of deficient skeletal growth in Raptor-deficient mice. These findings highlight a critical role of mTORC1 in mammalian skeletogenesis, at least in part, through translational control of Sox9 RNA. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2018 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | en |
dc.subject | mTORC1 | en |
dc.subject | translation | en |
dc.subject | Sox9 | en |
dc.subject | undifferentiated mesenchymal cells | en |
dc.title | Translational Control of Sox9 RNA by mTORC1 Contributes to Skeletogenesis | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Stem Cell Reports | en |
dc.identifier.volume | 11 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 228 | - |
dc.identifier.epage | 241 | - |
dc.relation.doi | 10.1016/j.stemcr.2018.05.020 | - |
dc.textversion | publisher | - |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School・Venture Business Laboratory, Organization of Frontier Science and Innovation, Kanazawa University | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.address | Institute for Virus Research, Kyoto University | en |
dc.address | Medical Research Institute, Kanazawa Medical University | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.address | Department of Neuroanatomy and Neuropharmacology, Faculty of Nursing, Chukyogakuin University | en |
dc.address | Medical Research Institute, Kanazawa Medical University | en |
dc.address | Institute for Virus Research, Kyoto University | en |
dc.address | Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School | en |
dc.identifier.pmid | 30008325 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 16H05131 | - |
datacite.awardNumber | 17KT0051 | - |
datacite.awardNumber | 18H04971 | - |
datacite.awardNumber | 17824969 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。