|Title:||Morphological changes of plasma membrane and protein assembly during clathrin-mediated endocytosis|
Yoshimura, Shige H.
|Author's alias:||吉田, 藍子|
|Publisher:||Public Library of Science (PLoS)|
|Journal title:||PLOS Biology|
|Abstract:||Clathrin-mediated endocytosis (CME) proceeds through a series of morphological changes of the plasma membrane induced by a number of protein components. Although the spatiotemporal assembly of these proteins has been elucidated by fluorescence-based techniques, the protein-induced morphological changes of the plasma membrane have not been fully clarified in living cells. Here, we visualize membrane morphology together with protein localizations during CME by utilizing high-speed atomic force microscopy (HS-AFM) combined with a confocal laser scanning unit. The plasma membrane starts to invaginate approximately 30 s after clathrin starts to assemble, and the aperture diameter increases as clathrin accumulates. Actin rapidly accumulates around the pit and induces a small membrane swelling, which, within 30 s, rapidly covers the pit irreversibly. Inhibition of actin turnover abolishes the swelling and induces a reversible open–close motion of the pit, indicating that actin dynamics are necessary for efficient and irreversible pit closure at the end of CME.|
|Description:||細胞が情報物質を取り込む瞬間の撮影に成功 --生きた細胞の表面を「見る」革新的技術--. 京都大学プレスリリース. 2018-07-26.|
|Rights:||© 2018 Yoshida et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
|Appears in Collections:||Journal Articles |
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