ダウンロード数: 145
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.stemcr.2018.03.016.pdf | 1.87 MB | Adobe PDF | 見る/開く |
タイトル: | FGF2 Has Distinct Molecular Functions from GDNF in the Mouse Germline Niche |
著者: | Masaki, Kaito Sakai, Mizuki Kuroki, Shunsuke Jo, Jun-Ichiro Hoshina, Kazuo Fujimori, Yuki Oka, Kenji Amano, Toshiyasu Yamanaka, Takahiro Tachibana, Makoto Tabata, Yasuhiko Shiozawa, Tanri Ishizuka, Osamu Hochi, Shinichi Takashima, Seiji |
著者名の別形: | 城, 潤一郎 |
キーワード: | fibroblast growth factor 2 glial cell line-derived neurotrophic factor retinoic acid spermatogonial differentiations permatogonial self-renewal germline niche |
発行日: | 5-Jun-2018 |
出版者: | Elsevier BV |
誌名: | Stem cell reports |
巻: | 10 |
号: | 6 |
開始ページ: | 1782 |
終了ページ: | 1792 |
抄録: | Both glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells, whereas retinoic acid (RA) induces spermatogonial differentiation. In this study, we investigated the functional differences between FGF2 and GDNF in the germline niche by providing these factors using a drug delivery system in vivo. Although both factors expanded the GFRA1⁺ subset of undifferentiated spermatogonia, the FGF2-expanded subset expressed RARG, which is indispensable for proper differentiation, 1.9-fold more frequently than the GDNF-expanded subset, demonstrating that FGF2 expands a differentiation-prone subset in the testis. Moreover, FGF2 acted on the germline niche to suppress RA metabolism and GDNF production, suggesting that FGF2 modifies germline niche functions to be more appropriate for spermatogonial differentiation. These results suggest that FGF2 contributes to induction of differentiation rather than maintenance of undifferentiated spermatogonia, indicating reconsideration of the role of FGF2 in the germline niche. |
著作権等: | © 2018 The Author(s).This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/233015 |
DOI(出版社版): | 10.1016/j.stemcr.2018.03.016 |
PubMed ID: | 29681540 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。