Downloads: 123

Files in This Item:
File Description SizeFormat 
s41598-018-28742-x.pdf4.28 MBAdobe PDFView/Open
Title: Live imaging analysis of the growth plate in a murine long bone explanted culture system
Authors: Hirota, Keisho
Yasoda, Akihiro
Kanai, Yugo
Ueda, Yohei  kyouindb  KAKEN_id
Yamauchi, Ichiro  kyouindb  KAKEN_id  orcid (unconfirmed)
Yamashita, Takafumi
Sakane, Yoriko
Fujii, Toshihito
Inagaki, Nobuya  kyouindb  KAKEN_id
Author's alias: 八十田, 明宏
金井, 有吾
山内, 一郎
坂根, 依利子
藤井, 寿人
稲垣, 暢也
Issue Date: 1-Dec-2018
Publisher: Nature Publishing Group
Journal title: Scientific Reports
Volume: 8
Thesis number: 10332
Abstract: Skeletal growth in mammals, which owes the growth of an individual, occurs at the growth plate and to observe and analyze its dynamic growth is of high interest. Here we performed live imaging analysis of the growth plate of a fetal murine long bone organ culture using two-photon excitation microscopy. We could observe a dynamic growth of the growth plate of explanted fetal murine ulna, as well as the resultant linear elongation of the explants. As for the factors contributing to the elongation of the growth plate, the displacement length of each chondrocyte was larger in the prehypertrophic or hypertrophic zone than in the proliferative zone. The segmented area and its extracellular component were increased in both the proliferative and prehypertrophic-hypertrophic zones, whereas an increase in cellular components was only seen in the prehypertrophic-hypertrophic zone. C-type natriuretic peptide, a known positive stimulator of endochondral bone growth mainly targeting prehypertrophic-hypertrophic zone, augmented all of the factors affecting growth plate elongation, whereas it had little effect on the proliferation of chondrocytes. Collectively, the axial trajectory of each chondrocyte mainly owes cellular or extracellular expansion especially in prehypertrophic-hypertrophic zone and results in growth plate elongation, which might finally result in endochondral bone elongation.
Rights: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
DOI(Published Version): 10.1038/s41598-018-28742-x
PubMed ID: 29985449
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks

Export Format: 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.