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Title: Development of new method to enrich human iPSC-derived renal progenitors using cell surface markers
Authors: Hoshina, Azusa
Kawamoto, Tatsuya
Sueta, Shin-Ichi
Mae, Shin-Ichi  kyouindb  KAKEN_id
Araoka, Toshikazu
Tanaka, Hiromi
Sato, Yasunori
Yamagishi, Yukiko
Osafune, Kenji  kyouindb  KAKEN_id
Author's alias: 保科, あずさ
前, 伸一
荒岡, 利和
長船, 健二
Issue Date: 23-Apr-2018
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 8
Thesis number: 6375
Abstract: Cell therapy using renal progenitors differentiated from human embryonic stem cells (hESCs) or induced pluripotent stem cells (hiPSCs) has the potential to significantly reduce the number of patients receiving dialysis therapy. However, the differentiation cultures may contain undifferentiated or undesired cell types that cause unwanted side effects, such as neoplastic formation, when transplanted into a body. Moreover, the hESCs/iPSCs are often genetically modified in order to isolate the derived renal progenitors, hampering clinical applications. To establish an isolation method for renal progenitors induced from hESCs/iPSCs without genetic modifications, we screened antibodies against cell surface markers. We identified the combination of four markers, CD9⁻CD140a⁺CD140b⁺CD271⁺, which could enrich OSR1⁺SIX2⁺ renal progenitors. Furthermore, these isolated cells ameliorated renal injury in an acute kidney injury (AKI) mouse model when used for cell therapy. These cells could contribute to the development of hiPSC-based cell therapy and disease modeling against kidney diseases.
Description: An Author Correction to this article was published on 18 July 2019
Rights: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/234234
DOI(Published Version): 10.1038/s41598-018-24714-3
PubMed ID: 29686294
Related Link: https://doi.org/10.1038/s41598-019-46254-0
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