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Title: Transient elevation of cytoplasmic calcium ion concentration at a single cell level precedes morphological changes of epidermal keratinocytes during cornification
Authors: Murata, Teruasa
Honda, Tetsuya
Egawa, Gyohei  kyouindb  KAKEN_id
Yamamoto, Yasuo
Ichijo, Ryo  kyouindb  KAKEN_id  orcid (unconfirmed)
Toyoshima, Fumiko  kyouindb  KAKEN_id
Dainichi, Teruki
Kabashima, Kenji
Author's alias: 村田, 光麻
本田, 哲也
江川, 形平
山本, 泰生
一條, 遼
豊島, 文子
大日, 輝記
椛島, 健治
Issue Date: 26-Apr-2018
Publisher: Springer Nature
Journal title: Scientific reports
Volume: 8
Thesis number: 6610
Abstract: Epidermal keratinocytes achieve sequential differentiation from basal to granular layers, and undergo a specific programmed cell death, cornification, to form an indispensable barrier of the body. Although elevation of the cytoplasmic calcium ion concentration ([Ca²⁺]i) is one of the factors predicted to regulate cornification, the dynamics of [Ca²⁺]i in epidermal keratinocytes is largely unknown. Here using intravital imaging, we captured the dynamics of [Ca²⁺]i in mouse skin. [Ca²⁺]i was elevated in basal cells on the second time scale in three spatiotemporally distinct patterns. The transient elevation of [Ca²⁺]i also occurred at the most apical granular layer at a single cell level, and lasted for approximately 40 min. The transient elevation of [Ca²⁺]i at the granular layer was followed by cornification, which was completed within 10 min. This study demonstrates the tightly regulated elevation of [Ca²⁺]i preceding the cornification of epidermal keratinocytes, providing possible clues to the mechanisms of cornification.
Rights: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
DOI(Published Version): 10.1038/s41598-018-24899-7
PubMed ID: 29700333
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