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タイトル: Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters
著者: Hata, Akihiko
Kitajima, Masaaki
Haramoto, Eiji
Lee, Suntae
Ihara, Masaru
Gerba, Charles P.
Tanaka, Hiroaki
著者名の別形: 端, 昭彦
李, 善太
井原, 賢
田中, 宏明
発行日: 7-Aug-2018
出版者: Springer Nature America, Inc
誌名: Scientific Reports
巻: 8
論文番号: 11837
抄録: Human astroviruses are associated with gastroenteritis and known to contaminate water environments. Three different genetic clades of astroviruses are known to infect humans and each clade consists of diverse strains. This study aimed to determine the occurrence and genetic diversity of astrovirus strains in water samples in different geographical locations, i.e., influent and effluent wastewater samples (n = 24 each) in Arizona, U.S., and groundwater (n = 37) and river water (n = 14) samples collected in the Kathmandu Valley, Nepal, using next-generation amplicon sequencing. Astrovirus strains including rare types (types 6 and 7 classical human astroviruses), emerging type (type 5 VA-astroviruses), and putative recombinants were identified. Feline astrovirus strains were collaterally identified and recombination between human and feline astroviruses was suggested. Classical- and VA-astroviruses seemed to be prevalent during cooler months, while MLB-astroviruses were identified only during warmer months. This study demonstrated the effectiveness of next-generation amplicon sequencing for identification and characterization of genetically diverse astrovirus strains in environmental water.
著作権等: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/234696
DOI(出版社版): 10.1038/s41598-018-30217-y
PubMed ID: 30087387
出現コレクション:学術雑誌掲載論文等

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