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Title: Light Control of the Tet Gene Expression System in Mammalian Cells
Authors: Yamada, Mayumi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-6272-3704 (unconfirmed)
Suzuki, Yusuke  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-5379-4030 (unconfirmed)
Nagasaki, Shinji C.
Okuno, Hiroyuki
Imayoshi, Itaru  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-9728-481X (unconfirmed)
Author's alias: 山田, 真弓
鈴木, 裕輔
長崎, 真治
奥野, 浩行
今吉, 格
Keywords: optogenetics
Tet system
Tet-OFF
Tet-ON
Cry2-CIB1
Issue Date: 9-Oct-2018
Publisher: Elsevier BV
Journal title: Cell Reports
Volume: 25
Issue: 2
Start page: 487
End page: 500.e6
Abstract: Gene expression and its network structure are dynamically altered in multicellular systems during morphological, functional, and pathological changes. To precisely analyze the functional roles of dynamic gene expression changes, tools that manipulate gene expression at fine spatiotemporal resolution are needed. The tetracycline (Tet)-controlled gene expression system is a reliable drug-inducible method, and it is used widely in many mammalian cultured cells and model organisms. Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution. By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system. This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics. We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
Description: 遺伝子のスイッチを「光」と「薬剤」で制御できる新技術を開発 --発生・幹細胞・神経科学研究への貢献に期待--. 京都大学プレスリリース. 2018-10-12.
Rights: © 2018 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/2433/234704
DOI(Published Version): 10.1016/j.celrep.2018.09.026
PubMed ID: 30304687
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2018/181010_1.html
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