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タイトル: | mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility |
著者: | Sakamoto, Satoko Thumkeo, Dean Ohta, Hiroshi Zhang, Zhen Huang, Shuangru Kanchanawong, Pakorn Fuu, Takayoshi Watanabe, Sadanori Shimada, Kentaro Fujihara, Yoshitaka Yoshida, Shosei Ikawa, Masahito Watanabe, Naoki https://orcid.org/0000-0001-8492-200X (unconfirmed) Saitou, Mitinori Narumiya, Shuh |
著者名の別形: | 坂本, 智子 タムケオ, ディーン 大田, 浩 島田, 健太郎 藤原, 祥高 吉田, 松生 伊川, 正人 渡邊, 直樹 斎藤, 通紀 成宮, 周 |
発行日: | 26-Sep-2018 |
出版者: | Public Library of Science (PLoS) |
誌名: | PLOS Biology |
巻: | 16 |
号: | 9 |
論文番号: | e2004874 |
抄録: | Formin is one of the two major classes of actin binding proteins (ABPs) with nucleation and polymerization activity. However, despite advances in our understanding of its biochemical activity, whether and how formins generate specific architecture of the actin cytoskeleton and function in a physiological context in vivo remain largely obscure. It is also unknown how actin filaments generated by formins interact with other ABPs in the cell. Here, we combine genetic manipulation of formins mammalian diaphanous homolog1 (mDia1) and 3 (mDia3) with superresolution microscopy and single-molecule imaging, and show that the formins mDia1 and mDia3 are dominantly expressed in Sertoli cells of mouse seminiferous tubule and together generate a highly dynamic cortical filamentous actin (F-actin) meshwork that is continuous with the contractile actomyosin bundles. Loss of mDia1/3 impaired these F-actin architectures, induced ectopic noncontractile espin1-containing F-actin bundles, and disrupted Sertoli cell–germ cell interaction, resulting in impaired spermatogenesis. These results together demonstrate the previously unsuspected mDia-dependent regulatory mechanism of cortical F-actin that is indispensable for mammalian sperm development and male fertility. |
記述: | 精子形態形成の分子メカニズムを超解像度顕微鏡と一分子イメージングで解明 --新しい男性不妊治療法の開発に向けて--. 京都大学プレスリリース. 2018-10-15. |
著作権等: | © 2018 Sakamoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/234707 |
DOI(出版社版): | 10.1371/journal.pbio.2004874 |
PubMed ID: | 30256801 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2018-10-15-2 |
出現コレクション: | 学術雑誌掲載論文等 |
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