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dc.contributor.author | Yamazaki, Shota | en |
dc.contributor.author | Harata, Masahiko | en |
dc.contributor.author | Idehara, Toshitaka | en |
dc.contributor.author | Konagaya, Keiji | en |
dc.contributor.author | Yokoyama, Ginji | en |
dc.contributor.author | Hoshina, Hiromichi | en |
dc.contributor.author | Ogawa, Yuichi | en |
dc.contributor.alternative | 小長谷, 圭志 | ja |
dc.contributor.alternative | 小川, 雄一 | ja |
dc.date.accessioned | 2018-10-23T01:27:15Z | - |
dc.date.available | 2018-10-23T01:27:15Z | - |
dc.date.issued | 2018-07-03 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2433/234722 | - |
dc.description.abstract | Polymerization of monomeric actin into filaments has pivotal roles in cell motility, growth, differentiation, and gene expression. Therefore, techniques of manipulating actin polymerization, including actin-binding chemicals, have been developed for understanding and regulating multiple biological functions. Here, we demonstrate that irradiation with terahertz (THz) waves is a novel method of modulating actin polymerization. When actin polymerization reaction is performed under irradiation with 0.46 THz waves generated by a Gyrotron, actin polymerization was observed to be activated by monitoring the fluorescence of pyrene actin fluorophores. We also observed the number of actin filaments under a fluorescence microscope using the polymerized actin probe SiR-actin. The number of actin filaments was increased by 3.5-fold after THz irradiation for 20 min. When the THz irradiation was applied to a steady-state actin solution, in which elongation and depolymerization of actin filaments were equilibrated, increased actin polymerization was observed, suggesting that the THz irradiation activates actin polymerization, at least in the elongation process. These results suggest that THz waves could be applied for manipulating biomolecules and cells. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Nature | en |
dc.rights | © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en |
dc.title | Actin polymerization is activated by terahertz irradiation | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Scientific Reports | en |
dc.identifier.volume | 8 | - |
dc.relation.doi | 10.1038/s41598-018-28245-9 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 9990 | - |
dc.address | Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University・Terahertz Sensing and Imaging Research Team, RIKEN Center for Advanced Photonics | en |
dc.address | Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University | en |
dc.address | Research Center for Development of Far-Infrared Region, University of Fukui (FIR UF) | en |
dc.address | Graduate School of Agriculture, Kyoto University | en |
dc.address | Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University | en |
dc.address | Terahertz Sensing and Imaging Research Team, RIKEN Center for Advanced Photonics | en |
dc.address | Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho | en |
dc.identifier.pmid | 29968762 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | JP25116009 | - |
datacite.awardNumber | JP15H04625 | - |
datacite.awardNumber | JP15K14706 | - |
datacite.awardNumber | JP16H05010 | - |
datacite.awardNumber | JP17H07365 | - |
datacite.awardNumber | JP17J00302 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
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