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Title: Significance of a histone-like protein with its native structure for the diagnosis of asymptomatic tuberculosis
Authors: Ohara, Yukiko
Ozeki, Yuriko
Tateishi, Yoshitaka
Mashima, Tsukasa
Arisaka, Fumio
Tsunaka, Yasuo
Fujiwara, Yoshie
Nishiyama, Akihito
Yoshida, Yutaka
Kitadokoro, Kengo
Kobayashi, Haruka
Kaneko, Yukihiro
Nakagawa, Ichiro  kyouindb  KAKEN_id
Maekura, Ryoji
Yamamoto, Saburo
Katahira, Masato  kyouindb  KAKEN_id
Matsumoto, Sohkichi
Author's alias: 大原, 由貴子
中川, 一路
片平, 正人
Keywords: General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Medicine
Issue Date: 25-Oct-2018
Publisher: Public Library of Science (PLoS)
Journal title: PLOS ONE
Volume: 13
Issue: 10
Thesis number: e0204160
Abstract: Tuberculosis causes the highest mortality among all single infections. Asymptomatic tuberculosis, afflicting one third of the global human population, is the major source as 5–10% of asymptomatic cases develop active tuberculosis during their lifetime. Thus it is one of important issues to develop diagnostic tools for accurately detecting asymptomatic infection. Mycobacterial DNA-binding protein 1 (MDP1) is a major protein in persistent Mycobacterium tuberculosis and has potential for diagnostic use in detecting asymptomatic infection. However, a previous ELISA-based study revealed a specificity problem; IgGs against MDP1 were detected in both M. tuberculosis-infected and uninfected individuals. Although the tertiary structures of an antigen are known to influence antibody recognition, the MDP1 structural details have not yet been investigated. The N-terminal half of MDP1, homologous to bacterial histone-like protein HU, is predicted to be responsible for DNA-binding, while the C-terminal half is assumed as totally intrinsically disordered regions. To clarify the relationship between the MDP1 tertiary structure and IgG recognition, we refined the purification method, which allow us to obtain a recombinant protein with the predicted structure. Furthermore, we showed that an IgG-ELISA using MDP1 purified by our refined method is indeed useful in the detection of asymptomatic tuberculosis.
Rights: © 2018 Ohara et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/2433/234938
DOI(Published Version): 10.1371/journal.pone.0204160
PubMed ID: 30359374
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