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journal.pone.0204964.pdf | 4.14 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Mano, Shoji | en |
dc.contributor.author | Nishihama, Ryuichi | en |
dc.contributor.author | Ishida, Sakiko | en |
dc.contributor.author | Hikino, Kazumi | en |
dc.contributor.author | Kondo, Maki | en |
dc.contributor.author | Nishimura, Mikio | en |
dc.contributor.author | Yamato, Katsuyuki T. | en |
dc.contributor.author | Kohchi, Takayuki | en |
dc.contributor.author | Nakagawa, Tsuyoshi | en |
dc.contributor.alternative | 西浜, 竜一 | ja |
dc.contributor.alternative | 石田, 咲子 | ja |
dc.contributor.alternative | 河内, 孝之 | ja |
dc.date.accessioned | 2018-11-05T02:43:18Z | - |
dc.date.available | 2018-11-05T02:43:18Z | - |
dc.date.issued | 2018-10-04 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2433/234942 | - |
dc.description.abstract | The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. polymorpha. The R4pMpGWB series allows tripartite recombination of any promoter and any coding sequence with a specific reporter or tag. Reporters/tags for the R4pMpGWB series are GUS, ELuc(PEST), FLAG, 3×HA, 4×Myc, mRFP1, Citrine, mCitrine, ER-targeted mCitrine and nucleus-targeted mCitrine. The R4L1pMpGWB series is suitable for promoter analysis. R4L1pMpGWB vector structure is the same as that of R4pMpGWB vectors, except that the attR2 site is replaced with attL1, enabling bipartite recombination of any promoter with a reporter or tag. Reporters/tags for the R4L1pMpGWB series are GUS, G3GFP-GUS, LUC, ELuc(PEST), Citrine, mCitrine, ER-targeted mCitrine and mCitrine-NLS. Both vector series were functional in M. polymorpha cells. These vectors will facilitate the design and assembly of plasmid constructs and generation of transgenic M. polymorpha. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | en |
dc.rights | © 2018 Mano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.title | Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | PLOS ONE | en |
dc.identifier.volume | 13 | - |
dc.identifier.issue | 10 | - |
dc.relation.doi | 10.1371/journal.pone.0204964 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e0204964 | - |
dc.address | Department of Cell Biology, National Institute for Basic Biology, Okazaki・Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies) | en |
dc.address | Graduate School of Biostudies, Kyoto University | en |
dc.address | Graduate School of Biostudies, Kyoto University | en |
dc.address | Department of Cell Biology, National Institute for Basic Biology, Okazaki | en |
dc.address | Spectrography and Bioimaging Facility, NIBB Core Research Facilities, National Institute for Basic Biology, Okazaki | en |
dc.address | Department of Cell Biology, National Institute for Basic Biology, Okazaki・Department of Biology, Faculty of Science and Engineering, Konan University | en |
dc.address | Faculty of Biology-Oriented Science and Technology, Kindai University | en |
dc.address | Graduate School of Biostudies, Kyoto University | en |
dc.address | Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization for Research, Shimane University | en |
dc.identifier.pmid | 30286137 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 26440157 | - |
datacite.awardNumber | 17K07457 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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