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dc.contributor.author | Frank, Viktoria | en |
dc.contributor.author | Kaufmann, Stefan | en |
dc.contributor.author | Wright, Rebecca | en |
dc.contributor.author | Horn, Patrick | en |
dc.contributor.author | Yoshikawa, Hiroshi Y. | en |
dc.contributor.author | Wuchter, Patrick | en |
dc.contributor.author | Madsen, Jeppe | en |
dc.contributor.author | Lewis, Andrew L. | en |
dc.contributor.author | Armes, Steven P. | en |
dc.contributor.author | Ho, Anthony D. | en |
dc.contributor.author | Tanaka, Motomu | en |
dc.contributor.alternative | 田中, 求 | ja |
dc.date.accessioned | 2018-11-26T02:19:41Z | - |
dc.date.available | 2018-11-26T02:19:41Z | - |
dc.date.issued | 2016-04-15 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2433/235331 | - |
dc.description.abstract | Mounting evidence indicated that human mesenchymal stem cells (hMSCs) are responsive not only to biochemical but also to physical cues, such as substrate topography and stiffness. To simulate the dynamic structures of extracellular environments of the marrow in vivo, we designed a novel surrogate substrate for marrow derived hMSCs based on physically cross-linked hydrogels whose elasticity can be adopted dynamically by chemical stimuli. Under frequent mechanical stress, hMSCs grown on our hydrogel substrates maintain the expression of STRO-1 over 20 d, irrespective of the substrate elasticity. On exposure to the corresponding induction media, these cultured hMSCs can undergo adipogenesis and osteogenesis without requiring cell transfer onto other substrates. Moreover, we demonstrated that our surrogate substrate suppresses the proliferation of hMSCs by up to 90% without any loss of multiple lineage potential by changing the substrate elasticity every 2nd days. Such “dynamic in vitro niche” can be used not only for a better understanding of the role of dynamic mechanical stresses on the fate of hMSCs but also for the synchronized differentiation of adult stem cells to a specific lineage. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Nature | en |
dc.rights | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en |
dc.title | Frequent mechanical stress suppresses proliferation of mesenchymal stem cells from human bone marrow without loss of multipotency | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Scientific Reports | en |
dc.identifier.volume | 6 | - |
dc.relation.doi | 10.1038/srep24264 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 24264 | - |
dc.address | Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg | en |
dc.address | Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg | en |
dc.address | Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg | en |
dc.address | Department of Medicine V, University of Heidelberg | en |
dc.address | Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg・Department of Chemistry, Saitama University | en |
dc.address | Department of Medicine V, University of Heidelberg | en |
dc.address | Department of Chemistry, Dainton Building, University of Sheffield | en |
dc.address | Biocompatibles UK Ltd | en |
dc.address | Department of Chemistry, Dainton Building, University of Sheffield | en |
dc.address | Department of Medicine V, University of Heidelberg | en |
dc.address | Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg・Institute for Integrated Cell-Material Sciences (WPI iCeMS), Kyoto University | en |
dc.identifier.pmid | 27080570 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 26103521 | - |
datacite.awardNumber | 26247070 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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