ダウンロード数: 164
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
ncomms12870.pdf | 596.67 kB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Hartel, Andreas J. W. | en |
dc.contributor.author | Glogger, Marius | en |
dc.contributor.author | Jones, Nicola G. | en |
dc.contributor.author | Abuillan, Wasim | en |
dc.contributor.author | Batram, Christopher | en |
dc.contributor.author | Hermann, Anne | en |
dc.contributor.author | Fenz, Susanne F. | en |
dc.contributor.author | Tanaka, Motomu | en |
dc.contributor.author | Engstler, Markus | en |
dc.contributor.alternative | 田中, 求 | ja |
dc.date.accessioned | 2018-11-26T02:30:18Z | - |
dc.date.available | 2018-11-26T02:30:18Z | - |
dc.date.issued | 2016-09-19 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/2433/235332 | - |
dc.description.abstract | The protein density in biological membranes can be extraordinarily high, but the impact of molecular crowding on the diffusion of membrane proteins has not been studied systematically in a natural system. The diversity of the membrane proteome of most cells may preclude systematic studies. African trypanosomes, however, feature a uniform surface coat that is dominated by a single type of variant surface glycoprotein (VSG). Here we study the density-dependence of the diffusion of different glycosylphosphatidylinositol-anchored VSG-types on living cells and in artificial membranes. Our results suggest that a specific molecular crowding threshold (MCT) limits diffusion and hence affects protein function. Obstacles in the form of heterologous proteins compromise the diffusion coefficient and the MCT. The trypanosome VSG-coat operates very close to its MCT. Importantly, our experiments show that N-linked glycans act as molecular insulators that reduce retarding intermolecular interactions allowing membrane proteins to function correctly even when densely packed. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Nature America, Inc | en |
dc.rights | © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en |
dc.title | N-glycosylation enables high lateral mobility of GPI-anchored proteins at a molecular crowding threshold | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Nature Communications | en |
dc.identifier.volume | 7 | - |
dc.relation.doi | 10.1038/ncomms12870 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 12870 | - |
dc.address | Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg・Department of Electrical Engineering, Bioelectronic Systems Lab, Columbia University | en |
dc.address | Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg | en |
dc.address | Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg | en |
dc.address | Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg | en |
dc.address | Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg | en |
dc.address | Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg | en |
dc.address | Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg | en |
dc.address | Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg・Institute for Integrated Cell-Material Science (WPI iCeMS), Kyoto University | en |
dc.address | Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg | en |
dc.identifier.pmid | 27641538 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。