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dc.contributor.author | Matsumiya, Marina | en |
dc.contributor.author | Tomita, Takehito | en |
dc.contributor.author | Yoshioka-Kobayashi, Kumiko | en |
dc.contributor.author | Isomura, Akihiro | en |
dc.contributor.author | Kageyama, Ryoichiro | en |
dc.contributor.alternative | 松宮, 舞奈 | ja |
dc.contributor.alternative | 吉岡-小林, 久美子 | ja |
dc.contributor.alternative | 磯村, 彰宏 | ja |
dc.contributor.alternative | 影山, 龍一郎 | ja |
dc.date.accessioned | 2018-11-26T04:04:30Z | - |
dc.date.available | 2018-11-26T04:04:30Z | - |
dc.date.issued | 2018-02-14 | - |
dc.identifier.issn | 0950-1991 | - |
dc.identifier.uri | http://hdl.handle.net/2433/235334 | - |
dc.description.abstract | Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7, which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | The Company of Biologists | en |
dc.rights | © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. | en |
dc.subject | Chemical library screening | en |
dc.subject | Embryonic stem cell | en |
dc.subject | Induced presomitic mesoderm | en |
dc.subject | Segmentation clock | en |
dc.subject | Self-organization | en |
dc.subject | Synchronized oscillation | en |
dc.title | ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Development | en |
dc.identifier.volume | 145 | - |
dc.identifier.issue | 4 | - |
dc.relation.doi | 10.1242/dev.156836 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | dev156836 | - |
dc.address | Institute for Frontier Life and Medical Sciences, Kyoto University・Graduate School of Biostudies, Kyoto University | en |
dc.address | Institute for Frontier Life and Medical Sciences, Kyoto University | en |
dc.address | Institute for Frontier Life and Medical Sciences, Kyoto University・Graduate School of Medicine, Kyoto University | en |
dc.address | Institute for Frontier Life and Medical Sciences, Kyoto University・Japan Science and Technology Agency, PRESTO | en |
dc.address | Institute for Frontier Life and Medical Sciences, Kyoto University・Graduate School of Biostudies, Kyoto University・Graduate School of Medicine, Kyoto University・Institute for Integrated Cell-Material Sciences, Kyoto University | en |
dc.identifier.pmid | 29437832 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 16H06480 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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