Access count of this item: 67
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
j.celrep.2018.07.056.pdf | 9.52 MB | Adobe PDF | View/Open |
Title: | Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents |
Authors: | Tainaka, Kazuki Murakami, Tatsuya C. Susaki, Etsuo A. Shimizu, Chika Saito, Rie Takahashi, Kei Hayashi-Takagi, Akiko Sekiya, Hiroshi Arima, Yasunobu Nojima, Satoshi Ikemura, Masako Ushiku, Tetsuo Shimizu, Yoshihiro Murakami, Masaaki Tanaka, Kenji F. Iino, Masamitsu Kasai, Haruo Sasaoka, Toshikuni Kobayashi, Kazuto Miyazono, Kohei Morii, Eiichi Isa, Tadashi Fukayama, Masashi Kakita, Akiyoshi Ueda, Hiroki R. |
Author's alias: | 伊佐, 正 |
Keywords: | chemistry in tissue clearing hydrophilic reagents high-throughput chemical screening comprehensive chemical profiling three-dimensional imaging virtual multiplex imaging |
Issue Date: | 21-Aug-2018 |
Publisher: | Elsevier BV |
Journal title: | Cell reports |
Volume: | 24 |
Issue: | 8 |
Start page: | 2196 |
End page: | 2210.e9 |
Abstract: | We describe a strategy for developing hydrophilic chemical cocktails for tissue delipidation, decoloring, refractive index (RI) matching, and decalcification, based on comprehensive chemical profiling. More than 1, 600 chemicals were screened by a high-throughput evaluation system for each chemical process. The chemical profiling revealed important chemical factors: salt-free amine with high octanol/water partition-coefficient (logP) for delipidation, N-alkylimidazole for decoloring, aromatic amide for RI matching, and protonation of phosphate ion for decalcification. The strategic integration of optimal chemical cocktails provided a series of CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis) protocols, which efficiently clear mouse organs, mouse body including bone, and even large primate and human tissues. The updated CUBIC protocols are scalable and reproducible, and they enable three-dimensional imaging of the mammalian body and large primate and human tissues. This strategy represents a future paradigm for the rational design of hydrophilic clearing cocktails that can be used for large tissues. |
Rights: | © 2018 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) |
URI: | http://hdl.handle.net/2433/235513 |
DOI(Published Version): | 10.1016/j.celrep.2018.07.056 |
PubMed ID: | 30134179 |
Appears in Collections: | Journal Articles |

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.