|Title:||Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents|
Murakami, Tatsuya C.
Susaki, Etsuo A.
Tanaka, Kenji F.
Ueda, Hiroki R.
|Author's alias:||伊佐, 正|
|Keywords:||chemistry in tissue clearing|
high-throughput chemical screening
comprehensive chemical profiling
virtual multiplex imaging
|Journal title:||Cell reports|
|Abstract:||We describe a strategy for developing hydrophilic chemical cocktails for tissue delipidation, decoloring, refractive index (RI) matching, and decalcification, based on comprehensive chemical profiling. More than 1, 600 chemicals were screened by a high-throughput evaluation system for each chemical process. The chemical profiling revealed important chemical factors: salt-free amine with high octanol/water partition-coefficient (logP) for delipidation, N-alkylimidazole for decoloring, aromatic amide for RI matching, and protonation of phosphate ion for decalcification. The strategic integration of optimal chemical cocktails provided a series of CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis) protocols, which efficiently clear mouse organs, mouse body including bone, and even large primate and human tissues. The updated CUBIC protocols are scalable and reproducible, and they enable three-dimensional imaging of the mammalian body and large primate and human tissues. This strategy represents a future paradigm for the rational design of hydrophilic clearing cocktails that can be used for large tissues.|
|Rights:||© 2018 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)|
|Appears in Collections:||Journal Articles |
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