このアイテムのアクセス数: 35

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
j.bbrep.2018.11.011.pdf1.24 MBAdobe PDF見る/開く
完全メタデータレコード
DCフィールド言語
dc.contributor.authorUmeda, Kentaro
dc.contributor.authorNegishi, Manabu
dc.contributor.authorKatoh, Hironori
dc.contributor.alternative梅田, 健太郎
dc.contributor.alternative根岸, 学
dc.contributor.alternative加藤, 裕教
dc.date.accessioned2018-12-13T00:38:22Z-
dc.date.available2018-12-13T00:38:22Z-
dc.date.issued2019-03
dc.identifier.issn2405-5808
dc.identifier.urihttp://hdl.handle.net/2433/235670-
dc.description.abstractThe appropriate development and regulation of neuronal morphology are important to establish functional neuronal circuits and enable higher brain function of the central nervous system. R-Ras, a member of the Ras family of small GTPases, plays crucial roles in the regulation of axonal morphology, including outgrowth, branching, and guidance. GTP-bound activated R-Ras reorganizes actin filaments and microtubules through interactions with its downstream effectors, leading to the precise control of axonal morphology. However, little is known about the upstream regulatory mechanisms for R-Ras activation in neurons. In this study, we found that brain-derived neurotrophic factor (BDNF) has a positive effect on endogenous R-Ras activation and promotes R-Ras-mediated axonal growth. RNA interference knockdown and overexpression experiments revealed that RasGRF1, a guanine nucleotide exchange factor (GEF) for R-Ras, is involved in BDNF-induced R-Ras activation and the promotion of axonal growth. Phosphorylation of RasGRF1 by protein kinase A at Ser916/898 is needed for the full activation of its GEF activity and to facilitate Ras signaling. We observed that BDNF treatment markedly increased this phosphorylation. Our results suggest that BDNF is one of the critical extrinsic regulators for R-Ras activation, and that RasGRF1 is an intrinsic key mediator for BDNF-induced R-Ras activation and R-Ras-mediated axonal morphological regulation.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier BV
dc.rights© 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
dc.subjectBrain-derived neurotrophic factor
dc.subjectR-Ras
dc.subjectRasGRF1
dc.subjectAxon
dc.titleRasGRF1 mediates brain-derived neurotrophic factor-induced axonal growth in primary cultured cortical neurons
dc.type.niitypeJournal Article
dc.identifier.jtitleBiochemistry and Biophysics Reports
dc.identifier.volume17
dc.identifier.spage56
dc.identifier.epage64
dc.relation.doi10.1016/j.bbrep.2018.11.011
dc.textversionpublisher
dc.identifier.kaken18K06215 / 17J02057
出現コレクション:学術雑誌掲載論文等

アイテムの簡略レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。