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Title: Loss of Stemness, EMT, and Supernumerary Tooth Formation in Cebpb⁻/⁻Runx2⁺/⁻ Murine Incisors
Authors: Saito, Kazuyuki
Saito, Kazuyuki
Takahashi, Katsu  kyouindb  KAKEN_id
Huang, Boyen
Asahara, Masakazu
Kiso, Honoka
Togo, Yumiko
Tsukamoto, Hiroko
Mishima, Sayaka  kyouindb  KAKEN_id
Nagata, Masaki
Iida, Machiko
Tokita, Yoshihito
Asai, Masato
Shimizu, Akira  kyouindb  KAKEN_id
Komori, Toshihisa
Harada, Hidemitsu
MacDougall, Mary
Sugai, Manabu
Bessho, Kazuhisa  kyouindb  KAKEN_id
Author's alias: 髙橋, 克
喜早, ほのか
清水, 章
別所, 和久
Issue Date: 26-Mar-2018
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 8
Thesis number: 5169
Abstract: Adult Cebpb KO mice incisors present amelogenin-positive epithelium pearls, enamel and dentin allopathic hyperplasia, fewer Sox2-positive cells in labial cervical loop epitheliums, and reduced Sox2 expression in enamel epithelial stem cells. Thus, Cebpb acts upstream of Sox2 to regulate stemness. In this study, Cebpb KO mice demonstrated cementum-like hard tissue in dental pulp, loss of polarity by ameloblasts, enamel matrix in ameloblastic layer, and increased expression of epithelial-mesenchymal transition (EMT) markers in a Cebpb knockdown mouse enamel epithelial stem cell line. Runx2 knockdown in the cell line presented a similar expression pattern. Therefore, the EMT enabled disengaged odontogenic epithelial stem cells to develop supernumerary teeth. Cebpb and Runx2 knockdown in the cell line revealed higher Biglycan and Decorin expression, and Decorin-positive staining in the periapical region, indicating their involvement in supernumerary tooth formation. Cebpb and Runx2 acted synergistically and played an important role in the formation of supernumerary teeth in adult incisors.
Rights: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
DOI(Published Version): 10.1038/s41598-018-23515-y
PubMed ID: 29581460
Appears in Collections:Journal Articles

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