Assessment of established techniques to determine developmental and malignant potential of human pluripotent stem cells

Allison, Thomas F.; Andrews, Peter W.; Avior, Yishai; Barbaric, Ivana; Benvenisty, Nissim; Bock, Christoph; Brehm, Jennifer; Brüstle, Oliver; Damjanov, Ivan; Elefanty, Andrew; Felkner, Daniel; Gokhale, Paul J.; Halbritter, Florian; Healy, Lyn E.; Hu, Tim X.; Knowles, Barbara B.; Loring, Jeanne F.; Ludwig, Tenneille E.; Mayberry, Robyn; Micallef, Suzanne; Mohamed, Jameelah S.; Müller, Franz-Josef; Mummery, Christine L.; Nakatsuji, Norio; Ng, Elizabeth S.; Oh, Steve K. W.; O’Shea, Orla; Pera, Martin F.; Reubinoff, Benjamin; Robson, Paul; Rossant, Janet; Schuldt, Bernhard M.; Solter, Davor; Sourris, Koula; Stacey, Glyn; Stanley, Edouard G.; Suemori, Hirofumi; Takahashi, Kazutoshi; Yamanaka, Shinya

このアイテムのアクセス数: 210

http://hdl.handle.net/2433/235744

このアイテムのファイル:

ファイル	記述	サイズ	フォーマット
s41467-018-04011-3.pdf		2.42 MB	Adobe PDF	見る/開く

完全メタデータレコード

DCフィールド	値	言語
dc.contributor.author	Allison, Thomas F.	en
dc.contributor.author	Andrews, Peter W.	en
dc.contributor.author	Avior, Yishai	en
dc.contributor.author	Barbaric, Ivana	en
dc.contributor.author	Benvenisty, Nissim	en
dc.contributor.author	Bock, Christoph	en
dc.contributor.author	Brehm, Jennifer	en
dc.contributor.author	Brüstle, Oliver	en
dc.contributor.author	Damjanov, Ivan	en
dc.contributor.author	Elefanty, Andrew	en
dc.contributor.author	Felkner, Daniel	en
dc.contributor.author	Gokhale, Paul J.	en
dc.contributor.author	Halbritter, Florian	en
dc.contributor.author	Healy, Lyn E.	en
dc.contributor.author	Hu, Tim X.	en
dc.contributor.author	Knowles, Barbara B.	en
dc.contributor.author	Loring, Jeanne F.	en
dc.contributor.author	Ludwig, Tenneille E.	en
dc.contributor.author	Mayberry, Robyn	en
dc.contributor.author	Micallef, Suzanne	en
dc.contributor.author	Mohamed, Jameelah S.	en
dc.contributor.author	Müller, Franz-Josef	en
dc.contributor.author	Mummery, Christine L.	en
dc.contributor.author	Nakatsuji, Norio	en
dc.contributor.author	Ng, Elizabeth S.	en
dc.contributor.author	Oh, Steve K. W.	en
dc.contributor.author	O’Shea, Orla	en
dc.contributor.author	Pera, Martin F.	en
dc.contributor.author	Reubinoff, Benjamin	en
dc.contributor.author	Robson, Paul	en
dc.contributor.author	Rossant, Janet	en
dc.contributor.author	Schuldt, Bernhard M.	en
dc.contributor.author	Solter, Davor	en
dc.contributor.author	Sourris, Koula	en
dc.contributor.author	Stacey, Glyn	en
dc.contributor.author	Stanley, Edouard G.	en
dc.contributor.author	Suemori, Hirofumi	en
dc.contributor.author	Takahashi, Kazutoshi	en
dc.contributor.author	Yamanaka, Shinya	en
dc.contributor.alternative	中辻, 憲夫	ja
dc.contributor.alternative	末盛, 博文	ja
dc.contributor.alternative	高橋, 和利	ja
dc.contributor.alternative	山中, 伸弥	ja
dc.date.accessioned	2018-12-21T02:53:40Z	-
dc.date.available	2018-12-21T02:53:40Z	-
dc.date.issued	2018-05-15	-
dc.identifier.issn	2041-1723	-
dc.identifier.uri	http://hdl.handle.net/2433/235744	-
dc.description.abstract	The International Stem Cell Initiative compared several commonly used approaches to assess human pluripotent stem cells (PSC). PluriTest predicts pluripotency through bioinformatic analysis of the transcriptomes of undifferentiated cells, whereas, embryoid body (EB) formation in vitro and teratoma formation in vivo provide direct tests of differentiation. Here we report that EB assays, analyzed after differentiation under neutral conditions and under conditions promoting differentiation to ectoderm, mesoderm, or endoderm lineages, are sufficient to assess the differentiation potential of PSCs. However, teratoma analysis by histologic examination and by TeratoScore, which estimates differential gene expression in each tumor, not only measures differentiation but also allows insight into a PSC’s malignant potential. Each of the assays can be used to predict pluripotent differentiation potential but, at this stage of assay development, only the teratoma assay provides an assessment of pluripotency and malignant potential, which are both relevant to the pre-clinical safety assessment of PSCs.	en
dc.format.mimetype	application/pdf	-
dc.language.iso	eng	-
dc.publisher	Springer Nature	en
dc.rights	© The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.	en
dc.title	Assessment of established techniques to determine developmental and malignant potential of human pluripotent stem cells	en
dc.type	journal article	-
dc.type.niitype	Journal Article	-
dc.identifier.jtitle	Nature Communications	en
dc.identifier.volume	9	-
dc.relation.doi	10.1038/s41467-018-04011-3	-
dc.textversion	publisher	-
dc.identifier.artnum	1925	-
dc.identifier.pmid	29765017	-
dcterms.accessRights	open access	-
出現コレクション:	学術雑誌掲載論文等

アイテムの簡略レコードを表示する

Export to RefWorks

このリポジトリに保管されているアイテムはすべて著作権により保護されています。