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タイトル: | Analysis of Ca²⁺ Response of Osteocyte Network by Three-dimensional Time-lapse Imaging in Living Bone |
著者: | Tanaka, Tomoyo Hoshijima, Mitsuhiro Sunaga, Junko Nishida, Takashi Hashimoto, Mana Odagaki, Naoya Osumi, Ryuta Adachi, Taiji https://orcid.org/0000-0001-5280-4156 (unconfirmed) Kamioka, Hiroshi |
著者名の別形: | 須長, 純子 安達, 泰治 |
キーワード: | Osteocyte MLO-Y4 Fluid shear stress 3D time-lapse imaging |
発行日: | Sep-2018 |
出版者: | Springer |
誌名: | Journal of Bone and Mineral Metabolism |
巻: | 36 |
号: | 5 |
開始ページ: | 519 |
終了ページ: | 528 |
抄録: | Osteocytes form a three-dimensional (3D) cellular network within the mineralized bone matrix. The cellular network has important roles in mechanosensation and mechanotransduction related to bone homeostasis. We visualized the embedded osteocyte network in chick calvariae and observed the flow-induced Ca²⁺ signaling in osteocytes using 3D time-lapse imaging. In response to the flow, intracellular Ca²⁺ ([Ca²⁺]i) significantly increased in developmentally mature osteocytes in comparison with young osteocytes in the bone matrix. To investigate the differences in response between young and developmentally mature osteocytes in detail, we evaluated the expression of osteocyte-related genes using the osteocyte-like cell line MLO-Y4, which was 3D-cultured within type I collagen gels. We found that the c-Fos, Cx43, Panx3, Col1a1, and OCN mRNA levels significantly increased on day 15 in comparison with day 7. These findings indicate that developmentally mature osteocytes are more responsive to mechanical stress than young osteocytes and have important functions in bone formation and remodeling. |
著作権等: | This is a post-peer-review, pre-copyedit version of an article published in Journal of Bone and Mineral Metabolism. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00774-017-0868-x. The full-text file will be made open to the public on 01 September 2019 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/235750 |
DOI(出版社版): | 10.1007/s00774-017-0868-x |
PubMed ID: | 29027020 |
出現コレクション: | 学術雑誌掲載論文等 |
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