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Title: Ethanol represses the expression of methanol-inducible genes via acetyl-CoA synthesis in the yeast Komagataella phaffii
Authors: Ohsawa, Shin
Nishida, Susumu
Oku, Masahide  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5991-6281 (unconfirmed)
Sakai, Yasuyoshi  kyouindb  KAKEN_id
Yurimoto, Hiroya  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7506-6184 (unconfirmed)
Author's alias: 大澤, 晋
奥, 公秀
阪井, 康能
由里本, 博也
Keywords: Applied microbiology
Cellular microbiology
Fungal genes
Issue Date: 21-Dec-2018
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 8
Start page: 18051
Abstract: In methylotrophic yeasts, the expression of methanol-inducible genes is repressed by ethanol even in the presence of methanol, a phenomenon called ethanol repression. The mechanism of ethanol repression in Komagataella phaffii (Pichia pastoris) was studied, and acetyl-CoA synthesis from ethanol by sequential reactions of alcohol dehydrogenase, aldehyde dehydrogenase and acetyl-CoA synthetase (ACS) was involved in ethanol repression. Molecular analysis of the ACS-encoding gene product KpAcs1 revealed that its N-terminal motif, which is conserved in methylotrophic yeasts, was required for ethanol repression. ACS activity was downregulated during methanol-induced gene expression, which partially depended on autophagy. In addition, acetyl-CoA synthesis and phosphorylation of a transcription factor KpMxr1 were found to contribute to ethanol repression in a synergistic manner.
Rights: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/235912
DOI(Published Version): 10.1038/s41598-018-36732-2
PubMed ID: 30575795
Appears in Collections:Journal Articles

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