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タイトル: Structural elucidation of a novel lipooligosaccharide from the Antarctic bacterium OMVs producer Shewanella sp. HM13
著者: Casillo, Angela
Di Guida, Rossella
Carillo, Sara
Chen, Chen
Kamasaka, Kouhei
Kawamoto, Jun
Kurihara, Tatsuo
Corsaro, Maria
著者名の別形: 陳, 晨
釜阪, 紘平
川本, 純
栗原, 達夫
キーワード: psychrophile
lipooligosaccharide (LOS)
structure elucidation
NMR spectroscopy
outer membrane vesicles (OMVs)
MALDI-TOF mass spectrometry
cold adaptation
発行日: Jan-2019
出版者: MDPI AG
誌名: Marine Drugs
巻: 17
号: 1
論文番号: 34
抄録: Shewanella sp. HM13 is a cold-adapted Gram-negative bacterium isolated from the intestine of a horse mackerel. It produces a large amount of outer membrane vesicles (OMVs), which are particles released in the medium where the bacterium is cultured. This strain biosynthesizes a single major cargo protein in the OMVs, a fact that makes Shewanella sp. HM13 a good candidate for the production of extracellular recombinant proteins. Therefore, the structural characterization of the components of the vesicles, such as lipopolysaccharides, takes on a fundamental role for understanding the mechanism of biogenesis of the OMVs and their applications. The aim of this study was to investigate the structure of the oligosaccharide (OS) isolated from Shewanella sp. HM13 cells as the first step for a comparison with that from the vesicles. The lipooligosaccharide (LOS) was isolated from dry cells, purified, and hydrolyzed by alkaline treatment. The obtained OS was analyzed completely, and the composition of fatty acids was obtained by chemical methods. In particular, the OS was investigated in detail by 1H and 13C NMR spectroscopy and MALDI-TOF mass spectrometry. The oligosaccharide was characterized by the presence of a residue of 8-amino-3, 8-dideoxy-manno-oct-2-ulosonic acid (Kdo8N) and of a d, d-heptose, with both residues being identified in other oligosaccharides from Shewanella species.
記述: This article belongs to the Special Issue Marine Bacteria as Sources of Bioactive Compounds.
著作権等: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/2433/236015
DOI(出版社版): 10.3390/md17010034
出現コレクション:学術雑誌掲載論文等

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