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2211-5463.12480.pdf | 1.29 MB | Adobe PDF | 見る/開く |
タイトル: | Co-translational folding of α-helical proteins: structural studies of intermediate-length variants of the λ repressor |
著者: | Hanazono, Yuya Takeda, Kazuki https://orcid.org/0000-0002-4094-6816 (unconfirmed) Miki, Kunio |
著者名の別形: | 花園, 祐矢 竹田, 一旗 三木, 邦夫 |
キーワード: | cotranslational folding crystal structure λ repressor |
発行日: | Aug-2018 |
出版者: | Wiley |
誌名: | FEBS Open Bio |
巻: | 8 |
開始ページ: | 1312 |
終了ページ: | 1321 |
抄録: | Nascent polypeptide chains fold cotranslationally, but the atomic‐level details of this process remain unknown. Here, we report crystallographic, de novo modeling, and spectroscopic studies of intermediate‐length variants of the λ repressor N‐terminal domain. Although the ranges of helical regions of the half‐length variant were almost identical to those of the full‐length protein, the relative orientations of these helices in the intermediate‐length variants differed. Our results suggest that cotranslational folding of the λ repressor initially forms a helical structure with a transient conformation, as in the case of a molten globule state. This conformation subsequently matures during the course of protein synthesis. |
著作権等: | © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/236159 |
DOI(出版社版): | 10.1002/2211-5463.12480 |
PubMed ID: | 30087834 |
出現コレクション: | 学術雑誌掲載論文等 |
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