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Title: Co-translational folding of α-helical proteins: structural studies of intermediate-length variants of the λ repressor
Authors: Hanazono, Yuya
Takeda, Kazuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Miki, Kunio
Author's alias: 花園, 祐矢
竹田, 一旗
三木, 邦夫
Keywords: cotranslational folding
crystal structure
λ repressor
Issue Date: Aug-2018
Publisher: Wiley
Journal title: FEBS Open Bio
Volume: 8
Start page: 1312
End page: 1321
Abstract: Nascent polypeptide chains fold cotranslationally, but the atomic‐level details of this process remain unknown. Here, we report crystallographic, de novo modeling, and spectroscopic studies of intermediate‐length variants of the λ repressor N‐terminal domain. Although the ranges of helical regions of the half‐length variant were almost identical to those of the full‐length protein, the relative orientations of these helices in the intermediate‐length variants differed. Our results suggest that cotranslational folding of the λ repressor initially forms a helical structure with a transient conformation, as in the case of a molten globule state. This conformation subsequently matures during the course of protein synthesis.
Rights: © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
DOI(Published Version): 10.1002/2211-5463.12480
PubMed ID: 30087834
Appears in Collections:Journal Articles

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