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dc.contributor.author | Loo, Yuet Ying | en |
dc.contributor.author | Rukayadi, Yaya | en |
dc.contributor.author | Nor-Khaizura, Mahmud-Ab-Rashid | en |
dc.contributor.author | Kuan, Chee Hao | en |
dc.contributor.author | Chieng, Buong Woei | en |
dc.contributor.author | Nishibuchi, Mitsuaki | en |
dc.contributor.author | Radu, Son | en |
dc.contributor.alternative | 西渕, 光昭 | ja |
dc.date.accessioned | 2019-02-08T06:43:45Z | - |
dc.date.available | 2019-02-08T06:43:45Z | - |
dc.date.issued | 2018-07-16 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/2433/236376 | - |
dc.description.abstract | Silver nanoparticles (AgNPs) used in this study were synthesized using pu-erh tea leaves extract with particle size of 4.06 nm. The antibacterial activity of green synthesized AgNPs against a diverse range of Gram-negative foodborne pathogens was determined using disk diffusion method, resazurin microtitre-plate assay (minimum inhibitory concentration, MIC), and minimum bactericidal concentration test (MBC). The MIC and MBC of AgNPs against Escherichia coli, Klebsiella pneumoniae, Salmonella Typhimurium, and Salmonella Enteritidis were 7.8, 3.9, 3.9, 3.9 and 7.8, 3.9, 7.8, 3.9 μg/mL, respectively. Time-kill curves were used to evaluate the concentration between MIC and bactericidal activity of AgNPs at concentrations ranging from 0×MIC to 8×MIC. The killing activity of AgNPs was fast acting against all the Gram-negative bacteria tested; the reduction in the number of CFU mL⁻¹ was >3 Log₁₀ units (99.9%) in 1–2 h. This study indicates that AgNPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agents for the treatment of bacterial infection including multidrug resistant bacterial infection. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media SA | en |
dc.rights | © 2018 Loo, Rukayadi, Nor-Khaizura, Kuan, Chieng, Nishibuchi and Radu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | en |
dc.subject | silver nanoparticles | en |
dc.subject | tea leave extracts | en |
dc.subject | antimicrobial activity | en |
dc.subject | foodborne pathogens | en |
dc.subject | Gram-negative | en |
dc.subject | time-kill curves | en |
dc.title | In Vitro Antimicrobial Activity of Green Synthesized Silver Nanoparticles Against Selected Gram-negative Foodborne Pathogens | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Frontiers in Microbiology | en |
dc.identifier.volume | 9 | - |
dc.relation.doi | 10.3389/fmicb.2018.01555 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 1555 | - |
dc.address | Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia | en |
dc.address | Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia | en |
dc.address | Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia | en |
dc.address | Department of Agricultural and Food Science, Faculty of Science, Universiti Tunku Abdul Rahman | en |
dc.address | Department of Chemistry, Faculty of Science, Universiti Putra Malaysia・Materials Processing and Technology Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia | en |
dc.address | Center for Southeast Asian Studies, Kyoto University | en |
dc.address | Department of Food Science, Faculty of Food Science and Technology・Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia | en |
dc.identifier.pmid | 30061871 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 24249038 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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