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dc.contributor.authorLoo, Yuet Yingen
dc.contributor.authorRukayadi, Yayaen
dc.contributor.authorNor-Khaizura, Mahmud-Ab-Rashiden
dc.contributor.authorKuan, Chee Haoen
dc.contributor.authorChieng, Buong Woeien
dc.contributor.authorNishibuchi, Mitsuakien
dc.contributor.authorRadu, Sonen
dc.contributor.alternative西渕, 光昭ja
dc.date.accessioned2019-02-08T06:43:45Z-
dc.date.available2019-02-08T06:43:45Z-
dc.date.issued2018-07-16-
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/2433/236376-
dc.description.abstractSilver nanoparticles (AgNPs) used in this study were synthesized using pu-erh tea leaves extract with particle size of 4.06 nm. The antibacterial activity of green synthesized AgNPs against a diverse range of Gram-negative foodborne pathogens was determined using disk diffusion method, resazurin microtitre-plate assay (minimum inhibitory concentration, MIC), and minimum bactericidal concentration test (MBC). The MIC and MBC of AgNPs against Escherichia coli, Klebsiella pneumoniae, Salmonella Typhimurium, and Salmonella Enteritidis were 7.8, 3.9, 3.9, 3.9 and 7.8, 3.9, 7.8, 3.9 μg/mL, respectively. Time-kill curves were used to evaluate the concentration between MIC and bactericidal activity of AgNPs at concentrations ranging from 0×MIC to 8×MIC. The killing activity of AgNPs was fast acting against all the Gram-negative bacteria tested; the reduction in the number of CFU mL⁻¹ was >3 Log₁₀ units (99.9%) in 1–2 h. This study indicates that AgNPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agents for the treatment of bacterial infection including multidrug resistant bacterial infection.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherFrontiers Media SAen
dc.rights© 2018 Loo, Rukayadi, Nor-Khaizura, Kuan, Chieng, Nishibuchi and Radu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.subjectsilver nanoparticlesen
dc.subjecttea leave extractsen
dc.subjectantimicrobial activityen
dc.subjectfoodborne pathogensen
dc.subjectGram-negativeen
dc.subjecttime-kill curvesen
dc.titleIn Vitro Antimicrobial Activity of Green Synthesized Silver Nanoparticles Against Selected Gram-negative Foodborne Pathogensen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleFrontiers in Microbiologyen
dc.identifier.volume9-
dc.relation.doi10.3389/fmicb.2018.01555-
dc.textversionpublisher-
dc.identifier.artnum1555-
dc.addressDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysiaen
dc.addressDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysiaen
dc.addressDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysiaen
dc.addressDepartment of Agricultural and Food Science, Faculty of Science, Universiti Tunku Abdul Rahmanen
dc.addressDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia・Materials Processing and Technology Laboratory, Institute of Advanced Technology, Universiti Putra Malaysiaen
dc.addressCenter for Southeast Asian Studies, Kyoto Universityen
dc.addressDepartment of Food Science, Faculty of Food Science and Technology・Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysiaen
dc.identifier.pmid30061871-
dcterms.accessRightsopen access-
datacite.awardNumber24249038-
jpcoar.funderName日本学術振興会ja
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
出現コレクション:学術雑誌掲載論文等

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