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Title: Recovery kinetics of micronucleus formation by fractionated X-ray irradiation in various types of human cells
Authors: Koyama, Shin  kyouindb  KAKEN_id
Narita, Eijiro
Shinohara, Naoki
Miyakoshi, Junji  kyouindb  KAKEN_id
Author's alias: 小山, 眞
成田, 英二郎
篠原, 真毅
宮越, 順二
Issue Date: Sep-2018
Publisher: Oxford University Press (OUP)
Journal title: Journal of radiation research
Volume: 59
Issue: 5
Start page: 547
End page: 554
Abstract: High-dose ionizing radiation is sufficient for breaking DNA strands, leading to cell death and mutations. By contrast, the effects of fractionated ionizing radiation on human-derived cells remain unclear. To better understand the genotoxic effects of fractionated ionizing radiation, as well as the cellular recovery rate, we investigated the frequency of micronucleus (MN) formation in various types of human cells. We irradiated cells with fractionated X-ray doses of 2 Gy at a rate of 0.0635 Gy/min, separated into two to eight smaller doses. After irradiation, we investigated the frequency of MN formation. In addition, we investigated the rate of decrease in MN frequency after irradiation with 1 or 2 Gy X-rays at various recovery periods. Fractionated irradiation decreased MN frequency in a dose-dependent manner. When the total dose of X-rays was the same, the MN frequencies were lower after fractionated X-ray irradiation than acute irradiation in every cell type examined. The rate of MN decrease was faster in KMST-6 cells, which were derived from a human embryo, than in the other cells. The rate of MN decrease was higher in cells exposed to fractionated X-rays than in those exposed to acute irradiation. Recovery rates were very similar among cell lines, except in KMST-6 cells, which recovered more rapidly than other cell types.
Rights: © The Author(s) 2018. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com)
URI: http://hdl.handle.net/2433/236391
DOI(Published Version): 10.1093/jrr/rry051
PubMed ID: 29961812
Appears in Collections:Journal Articles

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