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Title: The intracellular Ca²⁺ concentration is elevated in cardiomyocytes differentiated from hiPSCs derived from a Duchenne muscular dystrophy patient
Authors: Tsurumi, Fumitoshi
Baba, Shiro  kyouindb  KAKEN_id
Yoshinaga, Daisuke
Umeda, Katsutsugu
Hirata, Takuya
Takita, Junko  kyouindb  KAKEN_id  orcid (unconfirmed)
Heike, Toshio
Author's alias: 馬場, 志郎
吉永, 大介
梅田, 雄嗣
平田, 拓也
滝田, 順子
平家, 俊男
Keywords: General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Medicine
Issue Date: 15-Mar-2019
Publisher: Public Library of Science (PLoS)
Journal title: PLOS ONE
Volume: 14
Issue: 3
Thesis number: e0213768
Abstract: Duchenne muscular dystrophy (DMD) is the most common and severe form of muscular dystrophy. The major symptoms of this condition are walking difficulties, dyspnea caused by progressive skeletal muscle weakness, and cardiomyopathy. Recent advances in ventilator support devices have dramatically decreased mortality caused by respiratory distress. Consequently, cardiomyopathy resulting in heart failure is currently the major cause of death among DMD patients. One mechanism by which skeletal muscle is damaged in DMD patients involves elevation of the intracellular Ca²⁺ concentration. By contrast, the mechanisms underlying the development of cardiomyopathy are unclear. To investigate this, we examined the intracellular Ca²⁺ concentration and calcium transients in cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs). hiPSCs were derived from a DMD patient (DMD-hiPSCs), in whom exon 44 of the gene encoding dystrophin was deleted, and from his parents (control-hiPSCs), who did not carry this mutation. The intracellular Ca²⁺ concentration was measured using the fluorescent indicator indo-1. The fluorescence ratio (410/490 nm) of indo-1 at rest (R₀), the peak of this ratio (Rmax), and the amplitude (Rmax—R₀) were significantly higher in cardiomyocytes differentiated from DMD-hiPSCs than in those differentiated from control-hiPSCs. Moreover, mechanical stretching significantly increased the intracellular Ca²⁺ concentration in cardiomyocytes differentiated from DMD-hiPSCs, but not in those differentiated from control-hiPSCs. These findings indicate that elevation of the intracellular Ca²⁺ concentration can cause cardiac damage leading to cardiomyopathy in DMD patients.
Rights: © 2019 Tsurumi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI(Published Version): 10.1371/journal.pone.0213768
PubMed ID: 30875388
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