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Title: Analysis of genetic variation in mitochondrial cytochrome c oxidase subunit 1 between Haemadipsa japonica in Japan and land leeches worldwide
Authors: Sato, Naoe
Yokoyama, Chikako
Inukai, Miki
Miyashita, Saeko
Nagase, Keito
Nakano, Takafumi
Iuchi, Katsuya
Hisatomi, Hisashi
Author's alias: 中野, 隆文
Keywords: Mitochondrial DNA
Mitochondrial cox1
Haemadipsa japonica
the maximum likelihood method (ML) tree
Issue Date: 2019
Publisher: Informa UK Limited
Journal title: Mitochondrial DNA Part B
Volume: 4
Issue: 1
Start page: 1408
End page: 1410
Abstract: Haemadipsa japonica is the most common land leech species found in Japan. It has been considered to possess genetic variation that limits its habitat range. In the present study, to characterize variation in the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of H. japonica specimens from various locations in Japan, we examined nucleotide sequences of the mitochondrial cox1 gene. We performed PCR of mitochondrial cox1 using 10 H. japonica specimens and compared the result with those of land leeches from around the world using a maximum likelihood (ML) tree. ML tree of H. japonica in Japan showed significant differences between cox1 sequences of specimens from Yakushima and other regions of Japan. ML tree of land leeches from around the world revealed that H. japonica had the closest relationship with H. picta from Borneo.
Rights: © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/2433/240839
DOI(Published Version): 10.1080/23802359.2019.1598296
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