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ファイル | 記述 | サイズ | フォーマット | |
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j.virusres.2019.03.009.pdf | 954.29 kB | Adobe PDF | 見る/開く |
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DCフィールド | 値 | 言語 |
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dc.contributor.author | Kaido, Masanori | en |
dc.contributor.author | Nagano, Hideaki | en |
dc.contributor.author | Omote, Kazunori | en |
dc.contributor.author | Takano, Yoshitaka | en |
dc.contributor.author | Mise, Kazuyuki | en |
dc.contributor.author | Okuno, Tetsuro | en |
dc.contributor.alternative | 海道, 真典 | ja |
dc.contributor.alternative | 永野, 秀昭 | ja |
dc.contributor.alternative | 髙野, 義孝 | ja |
dc.contributor.alternative | 三瀬, 和之 | ja |
dc.contributor.alternative | 奥野, 哲郎 | ja |
dc.date.accessioned | 2019-04-23T06:17:37Z | - |
dc.date.available | 2019-04-23T06:17:37Z | - |
dc.date.issued | 2019-05 | - |
dc.identifier.issn | 0168-1702 | - |
dc.identifier.issn | 1872-7492 | - |
dc.identifier.uri | http://hdl.handle.net/2433/240991 | - |
dc.description.abstract | Carnation ringspot virus (CRSV) is the prototype virus of the genus Dianthovirus. Full-length cDNAs of CRSV strainsPV-0097 and PV-21 were constructed and the infectivity of in vitro transcripts was analyzed. Infectivity of PV-0097 and PV-21 to several plants was markedly higher than that of 1.30, a previously reported infectious CRSV clone. Overall RNA sequences of these viruses were similar, but PV-0097 and PV-21 contained additional nucleotides at the 5′ end of RNA1. Stem-loop structures were predicted in the 5′-terminal region of PV-0097 and PV-21 RNA1 but not in 1.30 RNA1. Mutant CRSV 1.30 RNA1 that contains the terminal 4 nucleotides of PV-0097, predicted to fold a 5′-terminal stem-loop structure, recovered higher level accumulation of viral RNAs in the inoculated protoplasts and leaves of Nicotiana benthamiana. These results suggest that the 5′-terminal stem-loop structure of CRSV RNA1 plays an important role in efficient amplification of the virus. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | en |
dc.rights | © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). | en |
dc.subject | Dianthovirus | en |
dc.subject | Carnation ringspot virus | en |
dc.subject | 5′-Terminal stem-loop | en |
dc.subject | Untranslated region | en |
dc.title | 5′-Terminal stem-loop of carnation ringspot virus RNA1 is required for the efficient amplification of viral RNAs | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Virus Research | - |
dc.identifier.volume | 265 | - |
dc.identifier.spage | 138 | - |
dc.identifier.epage | 142 | - |
dc.relation.doi | 10.1016/j.virusres.2019.03.009 | - |
dc.textversion | publisher | - |
dc.address | Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University | en |
dc.address | Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University | en |
dc.address | Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University | en |
dc.address | Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University | en |
dc.address | Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University | en |
dc.address | Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University | en |
dc.identifier.pmid | 30890436 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 16H04882 | - |
datacite.awardNumber | 15H04456 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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