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dc.contributor.authorKaido, Masanori
dc.contributor.authorNagano, Hideaki
dc.contributor.authorOmote, Kazunori
dc.contributor.authorTakano, Yoshitaka
dc.contributor.authorMise, Kazuyuki
dc.contributor.authorOkuno, Tetsuro
dc.contributor.alternative海道, 真典
dc.contributor.alternative永野, 秀昭
dc.contributor.alternative髙野, 義孝
dc.contributor.alternative三瀬, 和之
dc.contributor.alternative奥野, 哲郎
dc.date.accessioned2019-04-23T06:17:37Z-
dc.date.available2019-04-23T06:17:37Z-
dc.date.issued2019-05
dc.identifier.issn0168-1702
dc.identifier.issn1872-7492
dc.identifier.urihttp://hdl.handle.net/2433/240991-
dc.description.abstractCarnation ringspot virus (CRSV) is the prototype virus of the genus Dianthovirus. Full-length cDNAs of CRSV strainsPV-0097 and PV-21 were constructed and the infectivity of in vitro transcripts was analyzed. Infectivity of PV-0097 and PV-21 to several plants was markedly higher than that of 1.30, a previously reported infectious CRSV clone. Overall RNA sequences of these viruses were similar, but PV-0097 and PV-21 contained additional nucleotides at the 5′ end of RNA1. Stem-loop structures were predicted in the 5′-terminal region of PV-0097 and PV-21 RNA1 but not in 1.30 RNA1. Mutant CRSV 1.30 RNA1 that contains the terminal 4 nucleotides of PV-0097, predicted to fold a 5′-terminal stem-loop structure, recovered higher level accumulation of viral RNAs in the inoculated protoplasts and leaves of Nicotiana benthamiana. These results suggest that the 5′-terminal stem-loop structure of CRSV RNA1 plays an important role in efficient amplification of the virus.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier B.V.
dc.rights© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
dc.subjectDianthovirus
dc.subjectCarnation ringspot virus
dc.subject5′-Terminal stem-loop
dc.subjectUntranslated region
dc.title5′-Terminal stem-loop of carnation ringspot virus RNA1 is required for the efficient amplification of viral RNAs
dc.type.niitypeJournal Article
dc.identifier.jtitleVirus Research
dc.identifier.volume265
dc.identifier.spage138
dc.identifier.epage142
dc.relation.doi10.1016/j.virusres.2019.03.009
dc.textversionpublisher
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
dc.identifier.pmid30890436
dc.identifier.kaken16H04882 / 15H04456
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