Downloads: 199

Files in This Item:
File Description SizeFormat 
j.virusres.2019.03.009.pdf954.29 kBAdobe PDFView/Open
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKaido, Masanorien
dc.contributor.authorNagano, Hideakien
dc.contributor.authorOmote, Kazunorien
dc.contributor.authorTakano, Yoshitakaen
dc.contributor.authorMise, Kazuyukien
dc.contributor.authorOkuno, Tetsuroen
dc.contributor.alternative海道, 真典ja
dc.contributor.alternative永野, 秀昭ja
dc.contributor.alternative髙野, 義孝ja
dc.contributor.alternative三瀬, 和之ja
dc.contributor.alternative奥野, 哲郎ja
dc.date.accessioned2019-04-23T06:17:37Z-
dc.date.available2019-04-23T06:17:37Z-
dc.date.issued2019-05-
dc.identifier.issn0168-1702-
dc.identifier.issn1872-7492-
dc.identifier.urihttp://hdl.handle.net/2433/240991-
dc.description.abstractCarnation ringspot virus (CRSV) is the prototype virus of the genus Dianthovirus. Full-length cDNAs of CRSV strainsPV-0097 and PV-21 were constructed and the infectivity of in vitro transcripts was analyzed. Infectivity of PV-0097 and PV-21 to several plants was markedly higher than that of 1.30, a previously reported infectious CRSV clone. Overall RNA sequences of these viruses were similar, but PV-0097 and PV-21 contained additional nucleotides at the 5′ end of RNA1. Stem-loop structures were predicted in the 5′-terminal region of PV-0097 and PV-21 RNA1 but not in 1.30 RNA1. Mutant CRSV 1.30 RNA1 that contains the terminal 4 nucleotides of PV-0097, predicted to fold a 5′-terminal stem-loop structure, recovered higher level accumulation of viral RNAs in the inoculated protoplasts and leaves of Nicotiana benthamiana. These results suggest that the 5′-terminal stem-loop structure of CRSV RNA1 plays an important role in efficient amplification of the virus.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier B.V.en
dc.rights© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).en
dc.subjectDianthovirusen
dc.subjectCarnation ringspot virusen
dc.subject5′-Terminal stem-loopen
dc.subjectUntranslated regionen
dc.title5′-Terminal stem-loop of carnation ringspot virus RNA1 is required for the efficient amplification of viral RNAsen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleVirus Research-
dc.identifier.volume265-
dc.identifier.spage138-
dc.identifier.epage142-
dc.relation.doi10.1016/j.virusres.2019.03.009-
dc.textversionpublisher-
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Universityen
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Universityen
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Universityen
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Universityen
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Universityen
dc.addressLaboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Universityen
dc.identifier.pmid30890436-
dcterms.accessRightsopen access-
datacite.awardNumber16H04882-
datacite.awardNumber15H04456-
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
jpcoar.funderName.alternativeJapan Society for the Promotion of Science (JSPS)en
Appears in Collections:Journal Articles

Show simple item record

Export to RefWorks


Export Format: 


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.