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Title: Peptide barcoding for establishment of new types of genotype–phenotype linkages
Authors: Miyamoto, Kana
Aoki, Wataru
Ohtani, Yuta
Miura, Natsuko
Aburaya, Shunsuke
Matsuzaki, Yusei
Kajiwara, Kaho
Kitagawa, Yoshinori
Ueda, Mitsuyoshi
Author's alias: 宮本, 佳奈
青木, 航
大谷, 優太
三浦, 夏子
油屋, 駿介
松崎, 友星
梶原, 果穂
北川, 義康
植田, 充美
Issue Date: 23-Apr-2019
Publisher: Public Library of Science (PLoS)
Journal title: PLOS ONE
Volume: 14
Issue: 4
Thesis number: e0215993
Abstract: Measuring binding properties of binders (e.g., antibodies) is essential for developing useful experimental reagents, diagnostics, and pharmaceuticals. Display technologies can evaluate a large number of binders in a high-throughput manner, but the immobilization effect and the avidity effect prohibit the precise evaluation of binding properties. In this paper, we propose a novel methodology, peptide barcoding, to quantitatively measure the binding properties of multiple binders without immobilization. In the experimental scheme, unique peptide barcodes are fused with each binder, and they represent genotype information. These peptide barcodes are designed to have high detectability for mass spectrometry, leading to low identification bias and a high identification rate. A mixture of different peptide-barcoded nanobodies is reacted with antigen-coated magnetic beads in one pot. Peptide barcodes of functional nanobodies are cleaved on beads by a specific protease, and identified by selected reaction monitoring using triple quadrupole mass spectrometry. To demonstrate proof-of-principle for peptide barcoding, we generated peptide-barcoded anti-CD4 nanobody and anti-GFP nanobody, and determined whether we could simultaneously quantify their binding activities. We showed that peptide barcoding did not affect the properties of the nanobodies, and succeeded in measuring the binding activities of these nanobodies in one shot. The results demonstrate the advantages of peptide barcoding, new types of genotype–phenotype linkages.
Description: 多様なモノクロナル抗体分子を迅速に作製するペプチドバーコード手法を確立 --動物を使わずに試験管内で多様な抗体を調製することが可能に--. 京都大学プレスリリース. 2019-04-25.
Rights: © 2019 Miyamoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI(Published Version): 10.1371/journal.pone.0215993
PubMed ID: 31013333
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