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Title: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes.
Authors: Ito, Akira  kyouindb  KAKEN_id  orcid (unconfirmed)
Aoyama, Tomoki  kyouindb  KAKEN_id
Iijima, Hirotaka
Nishitani, Kohei  kyouindb  KAKEN_id
Tajino, Junichi
Kuroki, Hiroshi  kyouindb  KAKEN_id
Author's alias: 伊藤, 明良
青山, 朋樹
黒木, 裕士
Keywords: Heat stimuli
Extracellular matrix
Pellet culture
Issue Date: 14-Jan-2019
Publisher: Springer Nature
Journal title: BMC research notes
Volume: 12
Thesis number: 16
Abstract: [Objective]This study aimed to clarify the effects of periodic mild heat stimuli on extracellular matrix (ECM) synthesis of adult human chondrocytes in 3-dimensional pellet culture. [Results]Human articular chondrocytes were subjected to pellet culture at 37 °C for 3 days. Thereafter, the pellets were divided into three groups: 32 °C group which was cultured at 32 °C without heat stimuli, 32 °C + Heat group which was cultured at 32 °C and applied periodic heat stimuli, 37 °C group which was cultured at 37 °C. Heat stimuli were given by transferring the pellets into a CO2 incubator set at 41 °C for 20 min/day, 6 times/week. ECM synthesis ability was evaluated by analyzing the mRNA expressions. Additionally, the collagen and proteoglycan content in the pellet was quantified. DNA content was also measured for estimating the cell amount. We found that there were no significant differences in the mRNA expression of COL2A1, COL1A1, and ACAN between the 32 °C group and 32 °C + Heat group. However, the collagen content per cell and DNA content were significantly lower in the 32 °C + Heat group compared to other groups. Our results indicate that periodic mild heat stimuli may diminish ECM synthesis due to inhibition of collagen production and loss of cells.
Rights: © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
DOI(Published Version): 10.1186/s13104-019-4058-x
PubMed ID: 30642366
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