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Title: Identification of possible hypoxia sensor for behavioral responses in a marine annelid, Capitella teleta
Authors: Ogino, Tetsuya
Toyohara, Haruhiko
Author's alias: 荻野, 哲也
豊原, 治彦
Keywords: Hypoxia
Marine annelid
TRP channel
Behavior
Issue Date: 14-Mar-2019
Publisher: The Company of Biologists
Journal title: Biology Open
Volume: 8
Issue: 3
Thesis number: bio037630
Abstract: Hypoxia often occurs in summer and causes deleterious effects on marine benthic animals. A marine annelid, Capitella teleta, is tolerant to hypoxia, as shown by the fact that it inhabits organically polluted areas, where severe hypoxia is often observed. To understand how this species adapts to the environment, we focused on its hypoxia sensor, and we showed that TRPAbasal was a possible contributor to hypoxia detection in C. teleta. To examine the involvement of TRPA1 in the response of C. teleta to hypoxia, we exposed C. teleta to hypoxic water with or without a TRPA1-specific inhibitor, A-967079. Hypoxic stimulation induced escape behavior in C. teleta from the sediment, and this behavior was suppressed by the inhibitor. The cloned TRPA gene from C. teleta was phylogenetically categorized into TRPAbasal, and contains an oxygen-dependent degradation domain, which is important for the detection of hypoxia. Whole-mount in situ hybridization analysis showed that the gene was transcribed in the prostomium, where sensing functions are localized. These results suggested that the worm has a hypoxia-sensing system possibly utilizing CtTRPAbasal, and this system contributes to expanding the organism's niches in hypoxic environments by detecting whether hypoxia exceeds a level that would imperil its survival.
Rights: © 2019. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
URI: http://hdl.handle.net/2433/241697
DOI(Published Version): 10.1242/bio.037630
PubMed ID: 30745436
Appears in Collections:Journal Articles

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