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Title: Changes in the asymmetric distribution of cholesterol in the plasma membrane influence streptolysin O pore formation
Authors: Ogasawara, Fumihiko
Kano, Fumi
Murata, Masayuki
Kimura, Yasuhisa
Kioka, Noriyuki
Ueda, Kazumitsu
Author's alias: 小笠原, 史彦
加納, ふみ
村田, 昌之
木村, 泰久
木岡, 紀幸
植田, 和光
Keywords: Cytological techniques
Membrane lipids
Issue Date: 14-Mar-2019
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 9
Thesis number: 4548
Abstract: ATP-binding cassette A1 (ABCA1) plays a key role in generating high-density lipoprotein (HDL) and preventing atherosclerosis. ABCA1 exports cholesterol and phospholipid to apolipoprotein A-I (apoA-I) in serum to generate HDL. We found that streptolysin O (SLO), a cholesterol-dependent pore-forming toxin, barely formed pores in ABCA1-expressing cells, even in the absence of apoA-I. Neither cholesterol content in cell membranes nor the amount of SLO bound to cells was affected by ABCA1. On the other hand, binding of the D4 domain of perfringolysin O (PFO) to ABCA1-expressing cells increased, suggesting that the amount of cholesterol in the outer leaflet of the plasma membrane (PM) increased and that the cholesterol dependences of these two toxins differ. Addition of cholesterol to the PM by the MβCD–cholesterol complex dramatically restored SLO pore formation in ABCA1-expressing cells. Therefore, exogenous expression of ABCA1 causes reduction in the cholesterol level in the inner leaflet, thereby suppressing SLO pore formation.
Rights: © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/241744
DOI(Published Version): 10.1038/s41598-019-39973-x
PubMed ID: 30872611
Appears in Collections:Journal Articles

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