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タイトル: | The RECK tumor-suppressor protein binds and stabilizes ADAMTS10 |
著者: | Matsuzaki, Tomoko https://orcid.org/0000-0002-9878-0433 (unconfirmed) Kitayama, Hitoshi Omura, Akira Nishimoto, Emi Alexander, David B. Noda, Makoto https://orcid.org/0000-0002-7340-6066 (unconfirmed) |
著者名の別形: | 松﨑, 朋子 北山, 仁志 野田, 亮 |
キーワード: | RECK ADAMTS10 Tumor suppressor Fibronectin MT1-MMP Yeast two-hybrid assay |
発行日: | 4-Oct-2018 |
出版者: | Company of Biologists Ltd |
誌名: | Biology Open |
巻: | 7 |
論文番号: | bio033985 |
抄録: | The tumor suppressor protein RECK has been implicated in the regulation of matrix metalloproteinases (MMPs), NOTCH-signaling and WNT7-signaling. It remains unclear, however, how broad the spectrum of RECK targets extends. To find novel RECK binding partners, we took the unbiased approach of yeast two-hybrid screening. This approach detected ADAMTS10 as a RECK-interactor. ADAMTS10 has been characterized as a metalloproteinase involved in fibrillin-rich microfibril biogenesis, and its mutations have been implicated in the connective tissue disorder Weill-Marchesani syndrome. Experiments in vitro using recombinant proteins expressed in mammalian cells indicated that RECK indeed binds ADAMTS10 directly, that RECK protects ADAMTS10 from fragmentation following chemical activation and that ADAMTS10 interferes with the activity of RECK to inhibit MT1-MMP. In cultured cells, RECK increases the amount of ADAMTS10 associated with the cells. Hence, the present study has uncovered novel interactions between two molecules of known clinical importance, RECK and ADAMTS10. |
著作権等: | © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
URI: | http://hdl.handle.net/2433/242230 |
DOI(出版社版): | 10.1242/bio.033985 |
PubMed ID: | 30287421 |
出現コレクション: | 学術雑誌掲載論文等 |
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