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Title: The RECK tumor-suppressor protein binds and stabilizes ADAMTS10
Authors: Matsuzaki, Tomoko  kyouindb  KAKEN_id
Kitayama, Hitoshi
Omura, Akira
Nishimoto, Emi
Alexander, David B.
Noda, Makoto  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-7340-6066 (unconfirmed)
Author's alias: 松﨑, 朋子
北山, 仁志
野田, 亮
Keywords: RECK
ADAMTS10
Tumor suppressor
Fibronectin
MT1-MMP
Yeast two-hybrid assay
Issue Date: 4-Oct-2018
Publisher: Company of Biologists Ltd
Journal title: Biology Open
Volume: 7
Thesis number: bio033985
Abstract: The tumor suppressor protein RECK has been implicated in the regulation of matrix metalloproteinases (MMPs), NOTCH-signaling and WNT7-signaling. It remains unclear, however, how broad the spectrum of RECK targets extends. To find novel RECK binding partners, we took the unbiased approach of yeast two-hybrid screening. This approach detected ADAMTS10 as a RECK-interactor. ADAMTS10 has been characterized as a metalloproteinase involved in fibrillin-rich microfibril biogenesis, and its mutations have been implicated in the connective tissue disorder Weill-Marchesani syndrome. Experiments in vitro using recombinant proteins expressed in mammalian cells indicated that RECK indeed binds ADAMTS10 directly, that RECK protects ADAMTS10 from fragmentation following chemical activation and that ADAMTS10 interferes with the activity of RECK to inhibit MT1-MMP. In cultured cells, RECK increases the amount of ADAMTS10 associated with the cells. Hence, the present study has uncovered novel interactions between two molecules of known clinical importance, RECK and ADAMTS10.
Rights: © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
URI: http://hdl.handle.net/2433/242230
DOI(Published Version): 10.1242/bio.033985
PubMed ID: 30287421
Appears in Collections:Journal Articles

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